Asymmetrical hairpin probe and application thereof

Abstract

The invention relates to an asymmetrical hairpin probe and an application thereof, and belongs to the biological detection field. The asymmetrical hairpin probe consists of a target nucleotide distinguished sequence and a palindromic sequence; under the condition of no target sequence, the asymmetrical hairpin probe is under the metastable hairpin structure; once a target sequence to be detected is added, chain-type polymerization reaction free from the participation of enzyme is triggered; the target nucleic acid distinguished sequence part of the asymmetrical hairpin probe is combined with a corresponding target nucleic acid locus; rest probes are pairwise renatured into parts of double-chain probe of which the two ends are both provided with the palindromic sequence; and during molecular recognition hybridization, a detection signal is substantially enhanced so as to quickly detect the target sequence to be detected. According to the chain reaction technology of the asymmetrical hairpin probe, the high specificity of molecular hybridization and high efficiency of chain type polymerization are artfully combined, and free energy released by DNA (deoxyribose nucleic acid) polymerization is used for driving molecular cascade amplification. The asymmetrical hairpin probe has the advantages of simple reaction system and high detection efficiency, and the laboratory pollution problem existing in the traditional technology, such as PCR (polymerase chain reaction) and the like, can be effectively avoided.

Description

technical field [0001] The invention relates to a biological reaction technology, in particular to an asymmetric hairpin probe chain reaction technology. Background technique [0002] Nucleic acid molecular solid-phase hybridization, DNA sequencing, capillary electrophoresis analysis, gene chip technology and other detection methods have been established successively, which has raised the bacterial molecular detection method to a new stage, but most of these detection methods require special large-scale instruments and are technically difficult. It is difficult to popularize and apply in clinical practice. In addition, most samples need to be pre-amplified by PCR, which seriously interferes with the quantitative detection of pathogenic bacteria. At the same time, the laboratory pollution problem of PCR technology also greatly affects the accuracy of these detection methods. [0003] DNA probe technology, also known as molecular hybridization technology, is a new technology ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects in the above-mentioned fields, the present invention designs an asymmetric hairpin probe, and invents an asymmetric hairpin probe chain reaction technology, which skillfully combines the high specificity of molecular hybridization and the high efficiency of chain polymerization , the molecular cascade amplification is driven by the free energy released by DNA polymerization, the reaction system is simple, the detection efficiency is high, and the laboratory pollution problem existing in traditional techniques such as PCR is effectively avoided

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