Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit and method for detecting microRNA (ribonucleic acid)-155

A kit and asymmetric technology, applied in the field of kits for detecting microRNA-155

Inactive Publication Date: 2013-10-23
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no rapid, specific and convenient kit for detecting miR-155

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and method for detecting microRNA (ribonucleic acid)-155
  • Kit and method for detecting microRNA (ribonucleic acid)-155
  • Kit and method for detecting microRNA (ribonucleic acid)-155

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, hairpin probe and kit for detecting microRNA-155

[0029] 1. Selection of detection target sequences and design of asymmetric hairpin probes

[0030] (1) Select the target sequence for detection according to the molecular biological characteristics of miRNA.

[0031] through literature or Genbank databases ( http: / / www.ncbi.nlm.nih.gov / ) seized human sources related to cancer

[0032] miRNA sequence, which determines the detection target sequence.

[0033] (2) Design of specific asymmetric hairpin probes

[0034] Array Designer 4.0 software was used to design asymmetric hairpin probes for the target sequence, and the designed candidate probe sequences were submitted to the Genbank database for specificity analysis. Submit the designed asymmetric hairpin probes to The DINAMelt Web Server (http: / / mfold.rna.albany.edu / / ?q=DINAMelt / Hybrid2) for secondary structure analysis and between probes, between probes and The complementarity analysis of the target...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit and method for detecting microRNA (ribonucleic acid)-155. The kit comprises asymmetrical hairpin probes shown in SEQ ID NO.1 and SEQ ID NO.2. Compared with traditional RT-PCR (reverse transcription-polymerase chain reaction), northern blot and other technologies, the kit has the prominent advantages that (1) the kit carries out liquid-phase hybridization reaction, is closer to the natural state of molecular reaction, solves the problem of high steric hindrance of solid-phase hybridization, and improves the hybridization reaction efficiency; (2) the kit has high detection efficiency and high detection speed, and the traditional nucleic acid detection technology usually needs a few hours and even a dozen hours while the technology carries out molecular recognition and cascade signal amplification simultaneously, can complete nucleic acid detection within ten minutes and does not need to carry out RT-PCR on samples in advance; and (3) the reaction system is simple, dispenses with enzyme reaction and is low in cost.

Description

technical field [0001] The invention relates to the field of biology, in particular to a kit and method for detecting microRNA-155. Background technique [0002] microRNAs are a class of non-coding single-stranded small molecule RNAs found in a variety of eukaryotic cells and viruses on endogenous chromosomes, with a length of 21-25 nt, which are highly conserved in evolution and can pass through their targets. Complementation of the 3' untranslated region (3'UTR) of the mRNA molecule leads to inhibition of translation of the mRNA molecule. miRNA is involved in regulating the expression of more than one-third of eukaryotic genes, inhibiting the translation of target genes, causing mRNA degradation, etc., and has very important biological functions. Further studies have shown that miRNAs play key roles in cell proliferation, differentiation and apoptosis, neuronal polarity, insulin secretion, brain morphogenesis, heart formation, embryonic development, and individual death. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 夏涵梁盼盼黄庆府伟灵
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com