Preparation method of Hedyotis diffusa Willd. anti-cancer active component

A technology of Hedyotis diffusa and anticancer activity is applied in the directions of medical preparations, drug combinations, antitumor drugs, etc. containing active ingredients, which can solve the problems of staying, low extraction efficiency, long time consumption, etc. The effect of shortening the extraction time and using a small amount

Inactive Publication Date: 2013-03-13
牛宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are time-consuming and have low extraction efficiency
At present, the ethanol extraction process of Hedyotis diffusa only stays in the preparation stage of ethanol extract, and there is no report on the method of enriching the effective components in it by short column chromatography under reduced pressure

Method used

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  • Preparation method of Hedyotis diffusa Willd. anti-cancer active component
  • Preparation method of Hedyotis diffusa Willd. anti-cancer active component
  • Preparation method of Hedyotis diffusa Willd. anti-cancer active component

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for extracting anticancer active components of Hedyotis diffusa, comprising the following steps:

[0027] (1) The whole plant of Hedyotis diffusa is dried and crushed;

[0028] (2) At room temperature, 3 kg of Hedyotis diffusa was extracted twice with n-hexane ultrasonic assistance, and the n-hexane extract and residue were separated by gauze filtration and filter paper suction filtration, and the residue was evaporated to dry n-hexane. Add 15 L of n-hexane and extract for 40 minutes each time;

[0029] (3) At room temperature, the residue in step (2) was extracted twice with 90% ethanol ultrasonically, and the extract was filtered through gauze and filter paper successively, and 15 L of ethanol was added to each extraction, and 40 L was extracted each time. Minutes to obtain ethanol extract;

[0030] (4) Combine the two ethanol extracts and concentrate in vacuo at 40°C to obtain 253.28 g of extract;

[0031] (5) The extract was resuspended in 1000 mL of dei...

Embodiment 2

[0034] A method for extracting anticancer active components of Hedyotis diffusa, comprising the following steps:

[0035] (1) The whole plant of Hedyotis diffusa is dried and crushed;

[0036] (2) At room temperature, 3 kg of Hedyotis diffusa was extracted 3 times with the assistance of n-hexane ultrasound, and the n-hexane extract and residue were separated by gauze filtration and filter paper suction filtration, and the residue was evaporated to dry n-hexane. Add 30 L of n-hexane and extract for 60 minutes each time;

[0037] (3) At room temperature, the residue in step (2) was extracted three times with 95% ethanol ultrasonic assistance, the extract was filtered through gauze and filter paper successively, and 30 L of ethanol was added to each extraction, and 60 L was extracted each time. Minutes to obtain ethanol extract;

[0038] (4) Combine three ethanol extracts and concentrate in vacuo at 60°C to obtain 287.11 g of extract;

[0039] (5) The extract was resuspended i...

Embodiment 3

[0042] A method for extracting anticancer active components of Hedyotis diffusa, comprising the following steps:

[0043] (1) The whole plant of Hedyotis diffusa is dried and crushed;

[0044] (2) At room temperature, 3 kg of Hedyotis diffusa was extracted twice with n-hexane ultrasonic assistance, and the n-hexane extract and residue were separated by gauze filtration and filter paper suction filtration, and the residue was evaporated to dry n-hexane. Add 21 L of n-hexane and extract for 40 minutes each time;

[0045] (3) At room temperature, the residue in step (2) was extracted twice with 85% ethanol ultrasonically, and the extract was filtered through gauze and filter paper successively, and 21L of ethanol was added to each extraction, and each extraction was 50 minutes , to obtain ethanol extract;

[0046] (4) The two ethanol extracts were combined and concentrated in vacuo at 50°C to obtain 272.37 g of extract;

[0047] (5) The extract was resuspended in 1,000 mL of d...

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Abstract

The invention discloses an extraction method of a Hedyotis diffusa Willd. anti-cancer active component. The method includes: taking a Hedyotis diffusa Willd. whole plant as the raw material, extracting it 2-3 times with n-hexane, conducting pumping filtration to separate an n-hexane extraction solution and a residue, and extracting the residue 2-3 times with 85-95% ethanol so as to obtain ethanol extraction solutions; combining the ethanol extraction solutions, and carrying out concentration to obtain an extract; and re-suspending the extract in deionized water, then carrying out extraction with ether and ethyl acetate respectively, combining and concentrating the extracted substances, subjecting the mixture to silica gel column chromatography, performing gradient elution with n-hexane-dichloromethane and dichloromethane-ethyl acetate respectively, collecting the n-hexane-dichloromethane-ethyl acetate elution solution, and performing concentration and drying by evaporation, thus obtaining the Hedyotis diffusa Willd. extract. According to the invention, by means of ethanol solution extraction, liquid-liquid extraction enrichment and silica gel column chromatography purification, the method prepares the Hedyotis diffusa Willd. anti-cancer active component with increased content of free anthraquinone and total tritepenoidic acid, as well as substantially improved anticancer activity.

Description

technical field [0001] The invention relates to a method for extracting and enriching components of medicinal plants, in particular to a method for preparing anticancer active components of Hedyotis diffusa. Background technique [0002] In modern society, cancer is the second cause of death in developed countries (behind heart disease), and the third cause of death in developing countries (behind heart disease and intestinal infectious diseases). A major health problem that causes enormous physical and psychological suffering and economic burden to patients, families and society. As my country's population aging process accelerates, the incidence and mortality of cancer and other geriatric diseases are expected to increase. Therefore, it is of great significance to seek novel and effective cancer treatment drugs to alleviate the pain of patients and reduce the burden on families and society. [0003] Isolation of effective anticancer ingredients from traditional medicinal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/748A61P35/00
Inventor 蒙秋霞牛宇张丽珍牛伟
Owner 牛宇
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