Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
A technology of microbial inoculum and bio-organic fertilizer, applied in the field of bio-organic fertilizer and its preparation, special microbial inoculum for winter jujube, can solve problems such as short shelf life, and achieve the improvement of quality, production efficiency, and increase of ground diameter and seedling height. Effect
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Embodiment 1
[0038] Embodiment 1 is specially used in the preparation of the microbial bacterial agent of winter jujube, bio-organic fertilizer
[0039] The rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth of Dongzao rhizosphere was prepared according to the aforementioned cultivation method of the rhizosphere-promoting Bacillus subtilis DZ1.
[0040] Prepare the fermentation liquid of Saccharomyces cerevisiae and Lactobacillus casei according to the culture method provided by the strain preservation unit.
[0041]The obtained fermented liquid is uniformly mixed according to the quantity ratio of the number of living bacteria contained: the ratio of cfu of Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1, said Saccharomyces cerevisiae, and said Lactobacillus casei=10:1.5:2.5. The total number of viable bacteria in the bacterial liquid of the microbial composition is 3.36×10 8 cfu / ml.
[0042] The stabilizing aid is composed of the following components by we...
Embodiment 2
[0045] Embodiment 2 is specially used in the microbial bacterial agent of winter jujube
[0046] The rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth of Dongzao rhizosphere was prepared according to the aforementioned cultivation method of the rhizosphere-promoting Bacillus subtilis DZ1.
[0047] Prepare the fermentation liquid of Saccharomyces cerevisiae and Lactobacillus casei according to the culture method provided by the strain preservation unit.
[0048] The obtained fermented liquid is uniformly mixed according to the quantity ratio of the number of living bacteria contained: the ratio of the cfu of Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1, the Saccharomyces cerevisiae, and the Lactobacillus casei is 10:1.3:2.3. The total number of viable bacteria in the bacterial liquid of the microbial composition is 3.64×10 8 cfu / ml.
[0049] The stabilizing aid is composed of the following components by weight: 4.0 parts of calcium carbonate,...
Embodiment 3
[0050] Example 3 Application test of special microbial agent for winter jujube
[0051] The experiment was arranged in the experimental nursery of Shandong Academy of Forestry. Choose the year-old jujube seedlings with consistent growth, plant them in a plastic pot with a height of 20cm and a diameter of 30cm, get the special microbial inoculum for jujube made in Example 1 and dilute 10 times with tap water, get 100ml of the diluted inoculum (total effective live bacteria Number 40×10 8 cfu) was evenly watered around the roots of winter jujube seedlings, measured the dry weight of the aboveground part, the dry weight of the underground part, and the total dry weight (experimental group) when they grew for 6 months, and watered with 100ml of clear water (control group 1), and only watered the diluted Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth (total effective number of viable bacteria 40×10 8 cfu) 100ml (control group 2), only the diluted S....
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