Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof

A technology of microbial inoculum and bio-organic fertilizer, applied in the field of bio-organic fertilizer and its preparation, special microbial inoculum for winter jujube, can solve problems such as short shelf life, and achieve the improvement of quality, production efficiency, and increase of ground diameter and seedling height. Effect

Active Publication Date: 2013-03-13
SHANDONG FOREST SCI RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These applications are only preliminary, specifically as follows: (1) The microbial fertilizer produced by the above application only contains Bacillus subtilis DZ1, which promotes growth in the rhizosphere of winter jujube, and needs to add a variety of specific microbial populations to achieve the microbial cluster growth-promoting effect; ( 2) The fermentation conditions of Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1 need to be further optimized to realize industrial production; (3) The bacterial suspension is directly used as liquid fertilizer, and its shelf life is short, so appropriate stabilizers and protective agents need to be added to Protect the survival of live bacteria and extend the shelf life

Method used

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  • Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
  • Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
  • Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 is specially used in the preparation of the microbial bacterial agent of winter jujube, bio-organic fertilizer

[0039] The rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth of Dongzao rhizosphere was prepared according to the aforementioned cultivation method of the rhizosphere-promoting Bacillus subtilis DZ1.

[0040] Prepare the fermentation liquid of Saccharomyces cerevisiae and Lactobacillus casei according to the culture method provided by the strain preservation unit.

[0041]The obtained fermented liquid is uniformly mixed according to the quantity ratio of the number of living bacteria contained: the ratio of cfu of Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1, said Saccharomyces cerevisiae, and said Lactobacillus casei=10:1.5:2.5. The total number of viable bacteria in the bacterial liquid of the microbial composition is 3.36×10 8 cfu / ml.

[0042] The stabilizing aid is composed of the following components by we...

Embodiment 2

[0045] Embodiment 2 is specially used in the microbial bacterial agent of winter jujube

[0046] The rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth of Dongzao rhizosphere was prepared according to the aforementioned cultivation method of the rhizosphere-promoting Bacillus subtilis DZ1.

[0047] Prepare the fermentation liquid of Saccharomyces cerevisiae and Lactobacillus casei according to the culture method provided by the strain preservation unit.

[0048] The obtained fermented liquid is uniformly mixed according to the quantity ratio of the number of living bacteria contained: the ratio of the cfu of Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1, the Saccharomyces cerevisiae, and the Lactobacillus casei is 10:1.3:2.3. The total number of viable bacteria in the bacterial liquid of the microbial composition is 3.64×10 8 cfu / ml.

[0049] The stabilizing aid is composed of the following components by weight: 4.0 parts of calcium carbonate,...

Embodiment 3

[0050] Example 3 Application test of special microbial agent for winter jujube

[0051] The experiment was arranged in the experimental nursery of Shandong Academy of Forestry. Choose the year-old jujube seedlings with consistent growth, plant them in a plastic pot with a height of 20cm and a diameter of 30cm, get the special microbial inoculum for jujube made in Example 1 and dilute 10 times with tap water, get 100ml of the diluted inoculum (total effective live bacteria Number 40×10 8 cfu) was evenly watered around the roots of winter jujube seedlings, measured the dry weight of the aboveground part, the dry weight of the underground part, and the total dry weight (experimental group) when they grew for 6 months, and watered with 100ml of clear water (control group 1), and only watered the diluted Dongzao rhizosphere growth-promoting Bacillus subtilis DZ1 fermentation broth (total effective number of viable bacteria 40×10 8 cfu) 100ml (control group 2), only the diluted S....

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Abstract

The invention discloses microbial composition bacterial liquid comprising the components of winter jujube rhizosphere growth promoting bacillus subtilis DZ1, saccharomyces cerevisiae and lactobacillus casei. The invention also discloses a microbial agent special for winter jujube and a preparation method thereof. The microbial agent is formed in a way of mixing the microbial composition bacterial liquid and an effect stabilizing assistant according to the weight ratio of 100: (4-7). The invention additionally discloses a bio-organic fertilizer special for the winter jujube which is formed in way of mixing the microbial composition bacterial liquid and an organic fertilizer, wherein the ratio of the organic fertilizer to the microbial composition bacterial liquid is 10: (1-2)(W / V). Proved by experiments, compared with a winter jujube seedling which is singly inoculated by using three kinds of bacterial strain fermentation liquor or promotion stabilizing assistants, a winter jujube seedling which is inoculated by using the microbial agent disclosed by the invention has the advantages that the root weight, the ground diameter and the seedling height of the winter jujube are remarkably increased; and compared with a winter jujube fruiting tree which is applied by a bio-fertilizer formed in a way of inoculating the winter jujube rhizosphere growth promoting bacillus subtilis DZ1 into the organic fertilizer or the organic fertilizer, a winter jujube fruiting tree which is applied by the bio-organic fertilizer disclosed by the invention has the advantages that the yield is improved by 10-20% and the quality is obviously improved.

Description

technical field [0001] The invention relates to a special microbial agent for jujube, biological organic fertilizer and a preparation method thereof. Background technique [0002] The production management process of winter jujube is very complicated, and fertilization is one of the very important links. The fertilization effect is not only affected by the soil, the nutritional characteristics of the plant itself and the amount of fertilizer applied, but also related to the type of fertilizer. At present, the fertilizers used in winter jujube are mainly compound fertilizers composed of chemical fertilizers and organic fertilizers based on farmyard manure. The combined application of compound fertilizers and organic fertilizers improves the yield and quality of winter jujubes. Because the root system of winter jujube is underdeveloped, the growth is slow, the number of capillary roots is small, and the nutrient absorption capacity is poor, a large amount of compound fertiliz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05F11/08C12R1/125C12R1/865C12R1/245
Inventor 马海林邢尚军刘方春丁延琴杜秉海刘有东马丙尧陈波
Owner SHANDONG FOREST SCI RES INST
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