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A kind of arid mountain microbial combined bacterial liquid and its gel preparation

A gel preparation, microbial technology, applied in the application, fertilizer mixture, other chemical processes and other directions, can solve the problems of restricting the efficacy of microorganisms, limited water storage capacity of organic adsorption carriers, etc., to improve water use efficiency, improve afforestation effects, and improve effect of quantity

Active Publication Date: 2016-03-09
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the water storage capacity of organic adsorption carriers in bio-organic fertilizers is limited. In arid environments, affected by water stress, beneficial microorganisms in bio-organic fertilizers cannot reproduce successfully, which seriously restricts the effectiveness of microorganisms.

Method used

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  • A kind of arid mountain microbial combined bacterial liquid and its gel preparation
  • A kind of arid mountain microbial combined bacterial liquid and its gel preparation
  • A kind of arid mountain microbial combined bacterial liquid and its gel preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Preparation of microbial combination bacteria liquid and investigation of its influence on the adaptability of plants in arid environment

[0021] 1. Preparation of microbial combination bacteria liquid:

[0022] Bacillus cereus (Bacillus cereus) Z77, Bacillus cereus L90 and Saccharomyces cerevisiae were cultivated by seed culture and liquid fermentation culture according to conventional methods to prepare Bacillus cereus Z77 broth, Bacillus cereus L90 broth and winemaking Yeast broth.

[0023] Mix Bacillus cereus Z77, Bacillus cereus L90, and Saccharomyces cerevisiae in a cfu ratio of 10:4.7:0.7 to prepare a microbial combination liquid, which contains the number of viable cells 6.84×10 8 cfu / ml.

[0024] 2. The effect of microbial combination on the adaptability of plants in arid environment

[0025] The honeysuckle bare root seedlings were selected for pot experiment of honeysuckle bare root seedlings. When honeysuckle is planted, take 12ml of the prepared microb...

Embodiment 2

[0028] Example 2: Preparation of CLP-PGPR gel formulation and investigation of the survival of microbial combination bacteria in CLP type brake carrier

[0029] 1. Preparation of CLP-PGPR gel formulation

[0030] Boil soybean sprouts and water at a ratio of 1:30 (g / ml) for 10 minutes, cool and filter, add 1% (mass fraction) of glucose and 0.3% (mass fraction) of urea to the filtrate, and mix them after melting to prepare Into a special diluent. Mix Bacillus cereus Z77, Bacillus cereus L90, and Saccharomyces cerevisiae at the ratio of cfu: 10:4.5:0.8, and the microbial composition contains a viable cell count of 6.02×10 8 cfu / ml, respectively diluted 9 times with sterile water and special diluent.

[0031] Weigh 50g of CLP-type brake carrier, and mix the microbial composition and carrier with sterile water and special diluent respectively at a ratio of 25:1 (mL / g) to make CLP-PGPR gel preparation , Put it in a dry and cool place at room temperature, and store it naturally. After th...

Embodiment 3

[0032] Example 3: Afforestation test using CLP-PGPR gel formulation

[0033] The microbial adsorption solution diluted with the special diluent in Example 2 and the CLP-type brake carrier were used to prepare CLP-PGPR gel preparation, and the afforestation test of Platycladus orientalis was arranged. When Platycladus orientalis was planted, 125g CLP-PGPR gel preparation was taken, and Mix a small amount of soil and evenly spread it around the root system of honeysuckle bare root seedlings as the test group; take a CLP brake carrier of the same quality as the test group, mix it with a small amount of soil and evenly spread it around the root system of honeysuckle bare root seedlings. As a control group 1; take the same volume of microbial adsorption solution as the test group, mix it with a small amount of soil, and evenly spread it around the roots of the honeysuckle bare root seedlings, as a control group 2; take a CLP brake carrier of the same quality as the test group And the ...

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Abstract

The invention discloses a microorganism combined bacterium solution for drought mountain land. The microorganism combined bacterium solution is formed by mixing bacillus cereus Z77, bacillus cereus L90 and saccharomyces cerevisiae cfu in a ratio of 10: (4-5): (0.5-1), the viable count contained in the microorganism combined bacterium solution is greater than or equal to 5.0*108cfu / ml. The invention further discloses a gel preparation for the draught mountain land, the gel preparation is formed by mixing the microorganism adsorption liquid and an adsorption carrier in proportion of (20-30): 1, wherein the microorganism adsorption liquid is prepared by diluting the microorganism combined bacterium solution for 8-10 times by using a diluent solution. The microorganism combined bacterium solution realizes the cluster promotion effect under the draught environment of multi-strain, the combined bacterium solution has stronger pertinence and effectiveness to the drought environment; the gel preparation is capable of prolonging the survival time of the microorganism under the draught environment, the effective microorganism number is improved, the soil can be improved under the draught environment condition, the draught adaptive capacity of the plant is improved, and the survival rate of afforestation is improved.

Description

Technical field [0001] The invention relates to a microbial combined bacterial liquid for arid mountainous land and its gel preparation. Background technique [0002] Plant Rhizosphere Growth Promoting Bacteria (PGPR) refers to beneficial bacteria that live in the range of plant rhizosphere, promote plant growth or have antagonistic effects on pathogenic bacteria, and play an extremely important role in plant growth and disease control. At present, there are many research reports on PGPR, and a large amount of literature proves that PGPR can significantly promote the improvement of plant rhizosphere soil ecological environment, plant growth, especially plant root growth. [0003] Drought is a very serious natural disaster affecting agriculture and forestry production on a global scale. Drought will lead to a substantial decrease in the number of bacteria in the soil, a decrease in the diversity of community structure, and a decrease in soil biological activity, which will increase...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/00C09K105/00
CPCC09K17/00C09K2101/00
Inventor 马海林盛升井大炜刘方春马丙尧杜振宇邢尚军
Owner SHANDONG FOREST SCI RES INST
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