Duplex polymerase chain reaction (PCR) detection primer group, kit and method for pathogenic channa source aeromonas schubertii

A detection kit and technology for Aeromonas, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of missed treatment opportunities, easy misdiagnosis, etc., to avoid repeated cultivation and save Time-sensitive effects

Inactive Publication Date: 2014-02-26
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the disease is similar to the sarcoidosis caused by Nocardia, another major disease of the Channaidae fish, it is easy to misdiagnose and miss the best time for treatment during the breeding process.

Method used

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  • Duplex polymerase chain reaction (PCR) detection primer group, kit and method for pathogenic channa source aeromonas schubertii
  • Duplex polymerase chain reaction (PCR) detection primer group, kit and method for pathogenic channa source aeromonas schubertii
  • Duplex polymerase chain reaction (PCR) detection primer group, kit and method for pathogenic channa source aeromonas schubertii

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Experimental program
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Effect test

Embodiment 1

[0042] 1. Test strains

[0043]Channa-derived pathogenic Aeromonas schubert isolate WL-2 (for its isolation and identification methods, please refer to the literature: Isolation, identification and characteristic analysis of sarcoidosis-like pathogens in the viscera of hybrid snakeheads (Zhenna ♀×Brownia ♂)[ J], Liu Chun et al., Journal of Fisheries, 2012, Issue 07), Aeromonas sobria, Aeromonas hydrophila, Streptococcus agalactiae, Nocardia, Edwardsiella tarda, Pseudomonas fluorescens, columnar Flavobacterium, Vibrio harveyi, and Escherichia coli were isolated, identified and preserved by the inventor's laboratory (the Aquatic Disease and Immunity Laboratory of Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences).

[0044] 2. Culture of Bacteria

[0045] Aeromonas schubert isolate WL-2, Aeromonas sobria, Aeromonas hydrophila, Streptococcus agalactiae, Edwardsiella tarda, Pseudomonas fluorescens, P. Heart infusion agar plate was cultured at 28°C for ...

Embodiment 2

[0054] 1. Collection of disease data

[0055] Diseased fish were collected from Guangzhou and Foshan, and 21 strain samples were isolated.

[0056] 2. Disease detection

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Abstract

The invention discloses a duplex polymerase chain reaction (PCR) detection primer group, a kit and a method for pathogenic channa source aeromonas schubertii. Through two pairs of designed specific primers, a preferred reaction system and reaction conditions, pathogenic channa source aeromonas schubertii can be quickly and accurately detected, and a foundation for further researching the aeromonas schubertii and the pathogenesis thereof in laboratories is laid. The invention has the prominent advantages that a multiple PCR reaction is established, the sensitivity is strong, the specificity is high, pathogenic channa source aeromonas schubertii in a sample can be quickly and accurately detected, and at the same time, repeated cultivations and a series of lengthy biochemical reactions are avoided, so that time, labor and cost are reduced.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a double PCR detection primer set, kit and method for pathogenic snakehead-derived Aeromonas schubert. Background technique [0002] Channidae (Channidae) is an important freshwater economic fish in my country. It is widely distributed in my country. Its meat is tender, delicious, and has high nutritional value. Among them, Channa maculata and Channa argus and other Channa maculata ♀ × C. argus ♂ is an important cultured species in my country, and it is also an important freshwater economic fish for export in my country. With the development of high-density culture of pond snakeheads in my country, the problem of farmed snakehead fish diseases is becoming more and more serious, and the pathogens that lead to the occurrence of snakehead fish include Aeromonas hydrophila, Nocardia, Schubert Aeromonas etc. Channa viscera sarcoidosis is a new outbreak of bacterial vene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
Inventor 刘春李凯彬王庆吴淑勤王芳常藕琴梁慧丽曾伟伟王英英
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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