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Vaccines and methods for reducing campylobacter infection

A technology of bacteria and immunostimulatory polypeptides, applied in botany equipment and methods, biochemical equipment and methods, vaccines, etc., can solve the problem of no vaccine

Active Publication Date: 2015-11-25
阿肯色大学评议会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is currently no such vaccine

Method used

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  • Vaccines and methods for reducing campylobacter infection
  • Vaccines and methods for reducing campylobacter infection
  • Vaccines and methods for reducing campylobacter infection

Examples

Experimental program
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Embodiment

[0064] Attenuation of Salmonella candidate vaccine strains

[0065] Salmonella enteritidis phage type 13A (S. enteritidis) was attenuated by introducing defined, irreversible deletion mutations into the aroA and / or htrA genes of the Salmonella enteritidis genome as previously described (available at ATCC deposit numbers: PTA-7871, obtained by PTA-7872 and PTA-7873). Briefly, using kanamycin resistance (Km R ) gene sequence to replace the target gene sequence in the Salmonella Enteritidis bacterial genome. This step was performed using 3S-PCR and electroporating the 3S-PCR product into electrocompetent Salmonella cells containing the pKD46 plasmid. The resulting cell mixture was plated on LB agar plates supplemented with Km to select for the presence of Km R Gene positive clones. By making Km R There are sequences homologous to the target gene on both sides of the gene, and the Km R The gene is inserted into the genomic region containing the gene of interest (aroA or htrA...

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Abstract

Vaccine vectors and methods for enhancing resistance to Campylobacter infection or for enhancing the immune response to Campylobacter are provided herein. The vaccine vectors include a first polynucleotide which encodes an antigenic polypeptide selected from SEQ ID NO: 7-9 or a fragment thereof. The vector may also include an immunostimulatory polypeptide. The methods include administering the vaccine vectors to a subject.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to US Provisional Patent Application No. 61 / 353,039, filed June 9, 2010, which is incorporated herein by reference in its entirety. [0003] Statement Regarding Federally Sponsored Research [0004] This invention was made with government support under Grant No. 208-35-201-04-683 awarded by USDA / NRI. The government has certain rights in this invention. [0005] sequence listing [0006] This application has a Sequence Listing, which is hereby incorporated by reference in its entirety. A sequence listing was filed with the application as a text file on June 9, 2011. technical field [0007] The repertoire of safe and cost-effective vaccines for generating mucosal immunity against multiple agents is limited. The main bacterium causing worldwide human gastrointestinal diseases is Campylobacter. For the foreseeable future, bacterial gastroenteritis continues to pose a significant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02C12N1/20C12N15/00A61K35/74A61K39/00
CPCA61K39/12A61K39/105A61K2039/523A61K2039/53A61K2039/55516C07K14/205A61P31/04A61P37/04Y02A50/30A61K39/02A61K48/00C12N1/20C12N15/00C12N15/63C12N15/65
Inventor B·哈吉斯N·R·帕佛德Y·M·权S·莱顿
Owner 阿肯色大学评议会