Pesticide residue degradation agent taking chitosan oligosaccharide as active component and application thereof
A technology for the degradation of pesticide residues and active ingredients, applied in the fields of application, agriculture, and biocides, to achieve the effects of promoting plant growth, enhancing plant resistance, and being easy to popularize and apply
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Embodiment 1
[0020] Embodiment 1: Chitooligosaccharide directly degrades pesticide residue test
[0021] The chitosan oligosaccharide is formulated into an aqueous solution with a mass concentration of 0.1%, 0.01%, and 0.005%.
[0022] Mass concentration 20% fenpropathrin emulsifiable concentrate is diluted with water to the volume concentration 1:200 as stated on the label. Take 0.1mL of chitosan oligosaccharide solution of each concentration, add them to plastic centrifuge tubes with stoppers respectively, then add 1.0mL of fenpropathrin diluent, as a control, take 0.1mL of water and add 1.0mL of fenpropathrin diluent, mix well and react overnight .
[0023] Take 0.5ml of the reaction solution in each centrifuge tube, add 5.0mL of acetone, shake well, centrifuge at 2500r / min for 30min, suck out the supernatant with a dropper, add 3.0mL of dichloromethane, 10mL of sodium sulfate solution (5g / L) , vibrated for 1 min, and after standing to separate layers, move the dichloromethane extract...
Embodiment 2
[0027] Embodiment 2: Chitosan oligosaccharide degrades pesticide residue test in tobacco
[0028] Test crop: Potted tobacco.
[0029] Pesticide treatment: 5 groups were designed in the experiment, 20 tobacco plants in each group. 20% fenpropathrin emulsifiable concentrate was diluted with water to a volume concentration of 1:200 as stated on the label, and the tobacco leaves were sprayed with a dosage of 200mL. After 3 days of treatment, 3 groups were randomly selected for spraying chitosan oligosaccharides. The mass concentration of oligosaccharides was 0.1%, 0.05%, and 0.01% in turn. Only spraying and water treatment after spraying were used as two groups of controls, and the amount of chitosan and water treatment was also 200 mL for each group.
[0030] Sample treatment: After 3 days of chitosan oligosaccharide treatment, collect the tobacco leaves of each treatment group, take 250 grams of samples for each treatment group, after fully crushing, extract 2 times with 1.6L a...
Embodiment 3
[0034] Embodiment 3: Chitosan oligosaccharide degrades soil pesticide residue experiment
[0035] The test site in the greenhouse was selected for the experiment, and the medicament was carbofuran insecticide (3% granule in mass concentration), which belongs to carbamate pesticides. Experimental design 5 treatment groups, each treatment area is 2m 2 , 10g of medicine for each group, evenly sprinkled on the surface of the test ground, and turned the ground. The treatment groups were treated with chitosan oligosaccharides 3 days after the application of pesticides, and the treatment mass concentrations were 0.005%, 0.01%, 0.05% and 0.1%. The water treatment was used as the control group, and the ground was evenly sprayed with 1L per square meter. plow the ground. After 3 days of chitosan oligosaccharide treatment, samples were taken for detection.
[0036] Remove large pieces such as stones from the soil, pass through a 40-mesh sieve, and mix well. Weigh 20 grams of sample, ...
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