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Remediation method for copper and cadmium co-contaminated soil

A technology of compound pollution and repair method, which is applied in the field of ecological environmental protection and heavy metal polluted soil treatment, can solve the problems of low repair efficiency and rate, long growth cycle, slow growth, etc., achieve simple application method, increase biomass, and improve soil microorganisms volume effect

Active Publication Date: 2017-02-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although phytoremediation has great advantages in the remediation of heavy metal-contaminated soils, it also has limitations, such as low biomass, slow growth and long growth cycle of most heavy metal-accumulated plants, and low remediation efficiency and rate.

Method used

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  • Remediation method for copper and cadmium co-contaminated soil
  • Remediation method for copper and cadmium co-contaminated soil
  • Remediation method for copper and cadmium co-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Trichoderma viridans F7 fermented liquid combined with goosegrass repairs copper-cadmium composite polluted soil

[0030] The soil for testing was collected from polluted soil (sandy loam) in Dongli District, Tianjin City. Its basic physical and chemical properties are: pH 7.64 (soil-water ratio 1:2.5), organic matter 21.1g / kg, cation exchange capacity 15.9cmol / kg, total copper The content is 775mg / kg, and the total cadmium content is 3.05mg / kg. The soil samples were naturally air-dried, ground and passed through a 2mm (10 mesh) sieve, and used for pot plant simulation experiments.

[0031] The goosegrass seeds tested were purchased from the Chinese Academy of Agricultural Sciences.

[0032] The tested strain is Trichoderma virens strain F7 (preservation number: CGMCC No. 3.17613), the hyphae are white cotton flocculent and green spores.

[0033] Incline medium: adopt potato dextrose agar medium (PDA), weigh 200 grams of potatoes, wash and cut into pieces, ...

Embodiment 2

[0047] Example 2: Trichoderma viridans F7 solid preparation combined with goosegrass to remediate copper-cadmium composite polluted soil

[0048] The soil for testing was collected from polluted soil (silt soil) near the Jiuhuashan mining area in Nanjing City, Jiangsu Province. kg, the total copper content is 1200mg / kg, and the total cadmium content is 4.31mg / kg. The soil samples were naturally air-dried, ground and passed through a 2mm (10 mesh) sieve, and used for pot plant simulation experiments.

[0049] Goosegrass for testing, Trichoderma viridans F7, and culture medium are the same as in Example 1.

[0050] Preparation of Trichoderma viridans F7 fermentation broth: activate Trichoderma viridans F7 strains on PDA slant medium, cultivate them in the dark at 28°C for 5 days, wash the conidia with sterile water, and dilute the spore liquid to 2× 10 5 spores / mL was used as the seed solution for fermentation inoculation.

[0051] Wheat bran is used as the medium for the pr...

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Abstract

The invention relates to a remediation method for copper and cadmium co-contaminated soil. The method specifically comprises the steps that eleusine indica is planted in the contaminated soil, and Trichoderma virens F7 or inactivation Trichoderma virens F7 are applied in a combined manner; and the classification name of the Trichoderma virens F7 is Trichoderma virens, and is stored in the ordinary microorganism center of the Chinese microorganism culture preservation administration committee with the storage number of CGMCC No.3.17613. The contaminated soil is treated with the Trichoderma virens F7 combined with the eleusine indica; remediation efficiency for the copper and cadmium co-contaminated soil is improved; and a cooperative remediation effect improvement phenomenon exists between microorganisms and the plants; a good ecological effect is provided, and the remediation method is applicable to remediation of the copper and cadmium co-contaminated soil; the application method for Trichoderma virens F7 fermentation liquor and solid preparations is easy; the biomass of the eleusine indica is obviously increased; meanwhile, the microbial biomass of the soil can be increased; and the rhizosphere micro-ecological environment of the plants is improved.

Description

technical field [0001] The invention belongs to the field of ecological environmental protection, in particular to the field of heavy metal polluted soil treatment, and in particular relates to a repair method for copper-cadmium composite polluted soil. Background technique [0002] With the development of industry and the modernization of agricultural production, heavy metal pollution in farmland is becoming more and more serious, and the polluted area is expanding year by year. Heavy metals have attracted extensive attention due to their characteristics such as not easy to degrade and easy to enrich. Heavy metal pollution is mainly manifested in the compound pollution caused by the coexistence of various elements. Copper and cadmium pollution often exist in the soil at the same time, and both cadmium and excessive copper will affect the growth and development of crops, resulting in a decline in crop yield and quality, and through The food chain poses a hazard to human hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/105B09C2101/00
Inventor 宋宁宁刘君王凯荣咸洪泉刘亚男董瑞香李昌浩
Owner QINGDAO AGRI UNIV
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