Use of arnebia euchroma total polysaccharides in preparation of drug for preventing and treating acute lung injuries and acute respiratory distress syndrome

A technology for acute respiratory distress and acute lung injury, which is applied to total polysaccharide of comfrey, the preparation of medicines for preventing and treating acute lung injury and acute respiratory distress syndrome, and the new pharmaceutical application field of total polysaccharide from comfrey from traditional Chinese medicine, which can solve the problem of polysaccharide from comfrey in Xinjiang. There are few studies on pharmacological effects in vivo, and there are no reports on the prevention and treatment of ALI and antipyretic with total polysaccharides from Xinjiang comfrey, and the effects of improving hypoxia and pulmonary inflammation, reducing aggregation, and improving fever symptoms have been achieved.

Active Publication Date: 2013-04-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Throughout the research at home and abroad, there are few studies on the pharmacological effects of Xinjiang comfrey polysaccharides in vivo, and there are no reports on the prevention and treatment of ALI and antipyretic by Xinjiang comfrey polysaccharides

Method used

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  • Use of arnebia euchroma total polysaccharides in preparation of drug for preventing and treating acute lung injuries and acute respiratory distress syndrome
  • Use of arnebia euchroma total polysaccharides in preparation of drug for preventing and treating acute lung injuries and acute respiratory distress syndrome
  • Use of arnebia euchroma total polysaccharides in preparation of drug for preventing and treating acute lung injuries and acute respiratory distress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Take 100 g of the root medicinal materials of Arnebia euchroma (Royle) Johnst, pulverize, extract 3 times with 95% ethanol cold immersion, place the medicinal residues in a ventilated place at room temperature to dry, then extract 3 times with hot water, filter, combine The extract was concentrated, centrifuged, and the supernatant was removed with trichloroacetic acid to remove free protein; centrifuged, the supernatant was dialyzed with water for 3 days, the dialysate was concentrated to a small volume, ethanol was added to the alcohol content of 80%, centrifuged, and the precipitate was freeze-dried to obtain total polysaccharides. The sugar content of the total polysaccharides of Xinjiang comfrey was 75.31%-77.12% measured by the sulfuric acid-phenol method; the uronic acid content of the total polysaccharides of Xinjiang comfrey measured by the m-hydroxybiphenyl method was 22.58%-22.35%; the Coomassie Liang The protein content of the total polysaccharide of Xinjiang...

Embodiment 2

[0037] Forty-eight SD male rats were randomly divided into 6 groups (groups A, B, C, D, E, and F). Group A was the sham operation group, group B was the modeling group, and groups C, D, and E were the low-dose group (40 mg / kg, i.g.), the middle-dose group (80 mg / kg, i.g.), and the high-dose total polysaccharide group group (120 mg / kg, i.g.), group F was the positive control group (prednisone, hydroprednisone, 70 mg / kg, i.p.). 8 per group.

[0038] Except for the sham-operated group, the animals in the other groups were anesthetized by intraperitoneal injection of 1 g / kg urethane, and then fixed in a supine position. The right common carotid artery was isolated and cannulated for blood pressure monitoring, blood draw and reperfusion. After the animals were kept stable for 20 min, blood was drawn from the common carotid artery to a mean arterial pressure of 40±5 mmHg and maintained within 20 min, and then all the collected blood was slowly reinfused within 1 h after 1 h. The d...

Embodiment 3

[0046] There were 36 male Wistar rats (body weight 190±20 g). Before the experiment, the rats were acclimated to the experimental environment for at least 3 days, with free access to water and adaptive body temperature twice a day. Record the measured body temperature, exclude stress hyperthermia, and exclude those with large fluctuations in body temperature (with a difference of 0.3 °C for 2 consecutive times) or those with body temperature exceeding 38 °C. The day before the experiment, the rats were placed in specific rat cages with free access to water and fasted for 10 hours. The basal body temperature must be measured before the formal experiment begins. The body temperature of the experimental animals was measured 3 times with 15min interval each time, and the average of the body temperature of the 3 times was taken as the basal body temperature.

[0047] Animals were randomly divided into 6 groups (A, B, C, D, E, F groups). Group A was the normal control group, group...

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Abstract

The invention belongs to the field of traditional Chinese medicines, relates a novel pharmaceutical use of arnebia euchroma total polysaccharides from traditional Chinese medicines and especially relates to a use of arnebia euchroma total polysaccharides in preparation of a drug for preventing and treating acute lung injuries and acute respiratory distress syndrome. The invention realizes separation and extraction of total polysaccharides extract from a traditional Chinese medicine Xinjiang arnebia euchroma. The arnebia euchroma total polysaccharides obtained by the invention has a yield more than 3%, polysaccharides content more than 75%, uronic acid content more than 25% and protein content less than or equal to 10%. An overall animal model test proves that the Xinjing arnebia euchroma total polysaccharides has an obvious protection effect on LPS-induced acute lung injuries of rats and an obvious alleviation effect on LPS-induced rat fever, can be prepared into a drug for preventing and treating acute lung injuries and acute respiratory distress syndrome, and can be further used for preparation of a drug for preventing and treating severe SARS, a drug for bringing down a fever, a drug for preventing and treating human avian influenza, and a drug for preventing and treating A(H1N1)-type influenza, or a drug for preventing and treating seasonal influenza.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medicinal use of total polysaccharide from comfrey from traditional Chinese medicine. In particular, the invention relates to the use of total polysaccharide of comfrey in preparing medicines for preventing and treating acute lung injury and acute respiratory distress syndrome. Background technique [0002] Acute lung injury (ALI) refers to a combination of pulmonary inflammation and increased lung permeability caused by diffuse alveolar capillary membrane damage after the body is hit by severe infection, trauma, radiation, and shock. It is the early stage of acute respiratory distress syndrome (ARDS). The main pathophysiological changes are decreased lung compliance, increased intrapulmonary shunt, and imbalance of ventilation / blood flow. It is one of the more common clinical critical illnesses. The main manifestations are refractory hypoxemia, increased respira...

Claims

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Application Information

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IPC IPC(8): A61K36/30A61P11/00
Inventor 陈道峰姜韵章蕴毅
Owner FUDAN UNIV
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