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Alpha-amylase repressor SbAAI1 gene and protein as well as application of gene

An amylase inhibition and protein technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as affecting carbohydrate metabolism, deepening surface color, affecting processing quality, etc., to improve the ability to resist low-temperature saccharification and reduce the content of , The effect of improving the processing quality

Inactive Publication Date: 2014-03-05
HUAZHONG AGRI UNIV
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Problems solved by technology

However, low temperature affects the metabolism of carbohydrates in tubers and accelerates the degradation of starch into soluble reducing sugars. This phenomenon of "low temperature-induced tuber saccharification" is the main reason why most varieties are not suitable for processing
During the processing, the reducing sugar in the tuber and the α-amino acid of the nitrogen compound undergo "Maillard Reaction", which causes the surface color of potato chips or French fries to deepen, which seriously affects the processing quality

Method used

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  • Alpha-amylase repressor SbAAI1 gene and protein as well as application of gene
  • Alpha-amylase repressor SbAAI1 gene and protein as well as application of gene
  • Alpha-amylase repressor SbAAI1 gene and protein as well as application of gene

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Embodiment Construction

[0021] The present invention will be described in more detail below in conjunction with specific embodiments. From the following description and these Examples, one skilled in the art can ascertain the essential characteristics of the present invention. And without departing from the spirit and scope of the present invention, various changes and modifications can be made to the present invention to adapt it to various usages and conditions.

[0022] The amylase inhibitor gene of the present invention uses the wild species S. berthaultii resistant to low-temperature saccharification of potatoes as a material, and screens some genes related to low-temperature saccharification of potato tubers through gene chip differential hybridization technology. Using materials with different resistance to low-temperature saccharification, the expression profile of candidate genes was analyzed, and a gene whose expression level was closely related to the accumulation of reducing sugar after l...

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Abstract

The invention belongs to the technical field of plant genetic engineering and relates an alpha-amylase repressor SbAAI1 gene and protein as well as application of the gene in the aspect of increasing the low-temperature saccharification resistance capacity of potato tubers. The SbAAI1 gene related to the low-temperature saccharification resistance of the potato tubers is obtained by cloning; and the cDNA sequence of the gene is as shown in the SEQIDNO.1, and the protein sequence of the gene is as shown in the SEQIDNO.2. The genetic transformation shows that the gene can be applied to breeding and genetic improvement of the potato tubers for increasing the low-temperature saccharification resistance capacity, and a new gene resource is provided for potato plants.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to an alpha-amylase inhibitor SbAAI1 gene and protein, and an application thereof in improving the low-temperature saccharification resistance of potato tubers. Background technique [0002] potato( Solanum tuberosum L.) is the fourth largest food crop in my country, and it is also a vegetable, feed and industrial raw material crop. At the same time, my country is also the largest consumer of potatoes in the world. However, over the years, most of my country's potatoes have been used for fresh food and feed, and the processing share is less than 14%, while the processing utilization rate of major potato producing countries such as Europe and the United States is above 40%. With the development of my country's rural economy and the adjustment of industrial structure, the proportion of potatoes used as food is gradually decreasing, while the demand for fast food a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/21A01H5/00
Inventor 宋波涛张会灵谢从华刘勋柳俊
Owner HUAZHONG AGRI UNIV
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