Liposome including elastin-like polypeptide hydrophobic group conjugate, chemosensitizer and anticancer agent and use thereof

A technology of elastin and liposome, which is applied in the direction of liposome delivery, medical preparations containing active ingredients, and medical preparations with non-active ingredients, etc., which can solve problems such as limitations

Inactive Publication Date: 2013-04-24
SAMSUNG ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this technique is limited in cases where the phase transition temperature of liposomes is significantly higher than normal tissue temperature

Method used

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  • Liposome including elastin-like polypeptide hydrophobic group conjugate, chemosensitizer and anticancer agent and use thereof
  • Liposome including elastin-like polypeptide hydrophobic group conjugate, chemosensitizer and anticancer agent and use thereof
  • Liposome including elastin-like polypeptide hydrophobic group conjugate, chemosensitizer and anticancer agent and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Preparation of liposomes and introduction of drugs therein

[0078] Liposomes were prepared using the ingredients and composition ratios shown in Table 1 below.

[0079]

[0080]

[0081] *1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000](ammonium salt)

[0082] ** Stearoyl-VPGVG VPGVG VPGVG-NH 2 (SEQ ID NO:7, hereinafter referred to as SA-V3-NH 2 )

[0083] Specifically, the SA-V3-NH 2 Dissolve in ethanol, and dissolve DPPC (or DPPC / DSPC), DSPE-PEG and cholesterol in chloroform. After mixing the ethanol and chloroform solutions in the round bottom flask, the solvent was evaporated at room temperature under reduced pressure using a rotary evaporator, thereby forming a lipid thin layer on the inner wall of the flask.

[0084] Next, a 150 mM ammonium sulfate solution was added to the flask at room temperature to hydrate the lipid lamina. Vortex and sonicate the hydration solution. The resulting solution was trea...

Embodiment 2

[0100] Embodiment 2: Liposome loaded with doxorubicin and verapamil and its effect

[0101] Doxorubicin-resistant tumor cells, NCI / ADR-RES cells (from National Institute of Health (NIH)) were cultured in the presence of doxorubicin, and cell viability was evaluated. NCI / ADR-RES cells are tumor cells derived from OVCAR-8.

[0102] NCI / ADR-RES cells were treated with different concentrations of doxorubicin (0.1, 0.5, 1.0, 2.5, 5.0, 10 or 20ug / mL), 10% (volume percentage) fetal bovine serum (FBS), and 1% (weight percent) Penicillin-streptomycin (PS) was incubated in minimum essential medium (MEM) at 37°C for 2 hours. After replacing MEM with fresh medium, cells were incubated at 37°C for 2 days. Water soluble tetrazole (WST) assay to assess cell viability. figure 1 Shows the viability of NCI / ADR-RES cells when cultured in the presence of doxorubicin. Such as figure 1 As shown, more than about 70% of NCI / ADR-RES cells survived despite treatment with 20 ug / mL doxorubicin. ...

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Abstract

A liposome including a lipid bilayer, an elastin-like polypeptide (ELP) conjugated to a hydrophobic moiety; a chemosensitizer; and an anticancer agent, a pharmaceutical composition including the same, and a method of delivering a chemosensitizer and an anticancer agent to a target site of a subject by using the liposome.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of Korean Patent Application No. 10-2011-0107055 filed on October 19, 2011 and Korean Patent Application No. 10-2012-0108269 filed on September 27, 2012 at the Korean Intellectual Property Office, The disclosure thereof is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates to liposomes comprising elastin-like polypeptides coupled to hydrophobic group-containing moieties, chemosensitizing agents, and anticancer agents, pharmaceutical compositions comprising these liposomes, and Methods of using these liposomes to deliver chemosensitizers and anticancer agents to a target site in a subject. Background technique [0004] Liposomes have at least one lipid membrane enclosing an aqueous interior compartment. Liposomes can be identified by membrane type or by size. Small unilamellar vesicles (small unilamellar vesicles, SUVs) have a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/42A61P35/00
CPCA61K41/0028A61K41/0052A61K47/62A61K47/6911A61P35/00
Inventor 朴宣玟金泫伶金旻相朴在钻蔡洙荣
Owner SAMSUNG ELECTRONICS CO LTD
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