Unlock instant, AI-driven research and patent intelligence for your innovation.

Living cell detection device and detection method for tissue engineering reactor

A tissue engineering and detection device technology, applied in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, bioreactor/fermenter combinations, etc., can solve the continuous detection of cell growth and survival and Assess issues such as difficulty of implementation, scaffolding, and waste of seeded cell culture equipment

Inactive Publication Date: 2015-03-11
BEIHANG UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This results in a lot of waste of scaffolds, seeded cells, media and culture equipment
For the tissue culture process where the seed cells are scarce, the culture process is complicated, and the culture is difficult, the continuous detection and evaluation of cell growth and survival during the culture process is more difficult to achieve due to the limitation of the culture scale.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Living cell detection device and detection method for tissue engineering reactor
  • Living cell detection device and detection method for tissue engineering reactor
  • Living cell detection device and detection method for tissue engineering reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 Detects the amount of living cells on the cultured vascular tissue during tissue culture

[0064] (1) Rotate the three-way valve to switch the flow path, so that the tissue culture circuit upstream and downstream of the tissue culture chamber 105 is closed, the detection agent storage bottle 201 is closed, and the flushing circuit is unblocked;

[0065](2) Turn on the constant flow pump 103, so that the flushing liquid flows out from the flushing liquid storage bottle 101, flows through the tissue culture chamber 105, the detection chamber 107, and then flows into the waste liquid bottle, thereby completing the removal of the solution in the tissue culture chamber and the culture Replacement, to eliminate the interference of substances in the culture medium (such as exfoliated cells, etc.) on the detection;

[0066] (3) After flushing is completed, turn the three-way valve to switch the flow path, so that the flushing liquid storage bottle 101 is closed, th...

Embodiment 2

[0073] Embodiment 2 detects the cell viability on the microcarrier of suspension culture in tissue culture process

[0074] (1) Rotate the three-way valve to switch the flow path, so that the tissue culture circuit upstream and downstream of the tissue culture chamber 105 is closed, the detection agent storage bottle 201 is closed, and the flushing circuit is unblocked;

[0075] (2) Turn on the constant flow pump 103 to make the flushing liquid flow out from the flushing liquid storage bottle 101, flow through the tissue culture chamber 105, the cell trap 501 at the outlet of the tissue culture chamber, the detection chamber 107, and then flow into the waste liquid bottle, thus completing The replacement of the solution in the tissue culture chamber and the culture medium eliminates the interference of the substances in the culture medium on the detection;

[0076] (3) After flushing is completed, turn the three-way valve to switch the flow path, so that the flushing liquid st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a living cell detection device and a detection method for a tissue engineering reactor, which can detect the cell growth condition of tissue culture objects in the tissue engineering biological reactor under the preconditions of not damaging the cell activity and functions and not interrupting the cultivation process. The living cell detection device according to the embodiment of the invention comprises a flushing loop and a detection loop, wherein the flushing loop and the detection loop are in parallel connection with a culture loop of the tissue engineering reactor, and are combined with a pipe through a three-way valve; and the conversion of the loops is controlled through opening or closing a valve. The flushing loop comprises a flushing liquid storage bottle 101, a liquid driving pump 103 and a waste liquid bottle 109, and is used for flushing of tissue culture objects 111 before and after the detection; and the detection loop comprises a detection liquid storage bottle 201, the liquid driving pump 103, a detector 203, a computer 204 and the waste liquid bottle 109, and is used for detecting living cells on the tissue culture objects 111.

Description

technical field [0001] The present invention relates to the fields of cell culture and tissue engineering, more specifically to a detection device and detection method capable of detecting the growth of living cells on tissue culture or tissue implants in tissue engineering bioreactors, which are used for on-line or off-line continuous Quantitatively detect the real situation of cell growth and survival on a specific tissue engineering scaffold or tissue implant during tissue culture, evaluate the factors affecting cell growth and survival in tissue culture, and perform quality control on samples. Background technique [0002] An important application of tissue engineering technology is the construction of tissue implants in vitro, that is, the construction of tissue implants in vitro by tissue engineering methods is to seed cells cultured in vitro on natural or synthetic tissue engineering scaffolds, and after in vitro culture , and then transplanted into the body to replac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M3/02C12M1/34
Inventor 贡向辉樊瑜波
Owner BEIHANG UNIV