Cosmetic composition for improving skin elasticity
A cosmetic composition and skin-improving technology, applied in the direction of cosmetics, cosmetic preparations, skin care preparations, etc., can solve the problems of epidermis adhesion, such as few studies, to increase skin moisture content, improve skin wrinkles, and improve skin. elastic effect
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Embodiment 1
[0027] [Example 1] Increase the test of basement membrane glycan gene (isolation and RT-PCR of RNA)
[0028] Fibroblasts obtained from newborn infants at 1.25×10 6 The density of cells / dish was seeded onto 60-mm cell culture dishes using DMEM containing 10% serum and incubated at 37 °C in 5% CO 2 Grow to approximately 80% confluency in an incubator. After 24 hours of starvation, the cells washed twice with PBS (phosphate buffered solution) were treated with various concentrations of Phyllophyllum extract and ABcell TM processed and incubated for two days. After removing the medium, RNA was isolated by adding 1 mL of Trizol (Invitrogen) according to Invitrogen's RNA isolation method. After quantification of RNA at 260 nm using a UV detector (Hewlett Packard), reverse transcription-polymerase chain reaction (RT-PCR) was performed. The genetic analysis of each sample was corrected using the complementary 36B4 gene. The primer sequences for perlecan are as follows.
[0029] ...
Embodiment 2
[0032] [Example 2] Changes in perlecan staining by immunofluorescence
[0033] Normal human fibroblasts at 1.25×10 6 The density of cells / dish was seeded on 60-mm cell culture dishes using DMEM containing 10% serum and incubated at 37 °C in 5% CO 2 Grow to approximately 80% confluency in an incubator. After starvation for 24 hours, the cells were washed twice with PBS and cultured for 2 days while being irradiated with UV-B and treated with Phyllostachys radicis extract. Then, the growth of perlecan protein in the cellular state was investigated.
[0034] Adult dermal fibroblasts (HDFa) purchased from Cascade Biologics (USA) were incubated at 37°C in 5% CO using M106 medium (Cascade Biologics, USA). 2 cultivated in an incubator.
[0035] Immunofluorescent staining was carried out 48 hours after the cells were spotted on slides for immunofluorescent staining and treated with substances. Details about the immunofluorescent staining are as follows. The cells were rinsed twi...
Embodiment 3
[0038] [Example 3] by ABcellTM Test to restore perlecan levels
[0039] Normal human fibroblasts (NHF; isolated from 20- and 40-year-old adults) at 1.25×10 6 The density of cells / dish was seeded on 60-mm cell culture dishes using DMEM containing 10% serum and incubated at 37 °C in 5% CO 2 Grow to approximately 80% confluency in an incubator. The cultured cells were treated with 1% FBS medium + Cytokinol 100 ppm + 10% BASF for 48 hours and observed after perlecan staining. AB cells TM Treat at a concentration of 10ug / mL. The steps are similar to those in Example 4.
[0040] The results of the measured fluorescence intensity are shown in Table 1 and Figure 3-5 shown in . It was confirmed that perlecan levels in NHF of 40-year-old adults were reduced to less than those in NHF of 20-year-old adults ( image 3 and Figure 4 ), and the reduced perlecan levels in NHF of 40-year-old adults were restored to the levels of NHF of 20-year-old adults by HERA™ ABcell.
[0041] Tab...
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