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Method for fermenting acarbose

A fermentation method, acarbose technology, applied in the field of acarbose fermentation, can solve the problems of increased production cost, high energy consumption, increased stirring speed and ventilation speed, etc., to achieve low production cost, acarbose The effect of increased content and low price

Active Publication Date: 2014-07-16
SHANGHAI RECOM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method reduces the content of impurity A, the stirring speed and aeration speed are increased in the later stage of the method, and the duration is as long as 96h, which consumes a lot of energy and greatly increases the production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 The preparation method of acarbose of the present invention

[0028] (1) Seed culture: Use an inoculation loop to pick 2 rings of bacterial lawn from the slope to the shake flask culture medium, and place the shake flask in a constant temperature shaker at 28-32°C at 105-300rpm for shaking culture; after 24 hours, shake the flask Aseptically transfer the medium to the sterilized and cooled seed tank medium, stir at 200rpm, aeration at 0.15vvm, maintain the temperature at 28-32°C, and cultivate for 24h;

[0029] The ingredients and preparation method of shake flask seed medium are as follows: glycerin 0.5g / L, glucose 10g / L, soybean cake powder 5g / L, corn steep liquor 1g / L, calcium carbonate 0.5g / L, appropriate amount of purified water; add selenium-enriched yeast Powder 1g / L (it can also be replaced by other selenium-containing substances to ensure that the selenium content is 0.5mg / L); adjust the pH to 6.5 with 2mol / L sulfuric acid or sodium hydroxide solut...

Embodiment 2

[0044] Embodiment 2 The preparation method of acarbose of the present invention

[0045] (1) Seed culture: Use an inoculation loop to pick 1 ring of bacterial lawn from the slope to the shake flask culture medium, and place the shake flask in a constant temperature shaker at 28-32°C at 250rpm for shaking culture; after 28 hours, shake the flask culture medium Aseptically transfer to the sterilized and cooled seed tank culture medium, stir at 300rpm, aeration at 0.3vvm, maintain the temperature at 28-32°C, and cultivate for 20h;

[0046] Shake flask seed medium composition and preparation method: glycerin 1.0g / L, glucose 12.5g / L, soybean cake powder 15g / L, corn steep liquor 1.5g / L, calcium carbonate 1g / L, appropriate amount of purified water; add selenium-enriched yeast Powder 1-3g / L (it can also be replaced by other selenium-containing substances to ensure that the selenium content is 1.0mg / L); adjust the pH to 7.5 with 2mol / L sodium hydroxide solution; sterilize with pure ste...

Embodiment 3

[0061] Embodiment 3 The preparation method of acarbose of the present invention

[0062] (1) Seed culture: Use an inoculation loop to pick 3 rings of bacterial lawn from the slope to the shake flask medium, and place the shake flask in a constant temperature shaker at 28-32°C at 280rpm for shaking culture; after 24 hours, shake the flask culture medium Aseptically transfer to the sterilized and cooled seed tank culture medium, stir at 300rpm, aeration at 0.3vvm, maintain the temperature at 28-32°C, and cultivate for 20h;

[0063] Shake flask seed medium composition and preparation method: glycerin 2.0g / L, glucose 20g / L, soybean cake powder 15g / L, corn steep liquor 2g / L, calcium carbonate 1g / L, appropriate amount of purified water; add selenium-enriched yeast powder 1 -3g / L (It can also be replaced by other selenium-containing substances to ensure that the selenium content is 1.5mg / L); adjust the pH to 7.0 with 2mol / L sodium hydroxide solution; sterilize with pure steam at 121°...

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PUM

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Abstract

The invention discloses a method for fermenting acarbose. The method comprises the following steps of: (1) taking Actinoplanes species and inoculating into actinomyces seed culture medium to prepare seed solution; and (2) taking the seed solution prepared in the step (1), inoculating into an actinomyce fermentation medium, and fermenting, thereby obtaining acarbose fermentation broth, wherein selenium with the concentration of 2-21mg / L is added in the fermentation medium. The fermentation method disclosed by the invention has the advantages of increasing the yield of the acarbose, remarkably reducing the content of an impurity A in the acarbose and shortening the fermentation period.

Description

technical field [0001] The invention relates to a fermentation method of acarbose. Background technique [0002] Acarbose is an oligosaccharide composed of four monosaccharides containing an unsaturated cyclic polyol, chemical name: 0-4,6-dideoxy-4-[(1S,4R,5S,6S) 4,5,6-Trihydroxy-3-(hydroxymethyl)-2-cyclohexenyl-1-amino]-α-D-glucopyranosyl-(1,4)-0-α-D -Glucopyranosyl-(1,4)-D-glucopyranose. Acarbose is a new type of oral hypoglycemic drug that competitively inhibits glucoside hydrolase in the intestinal tract, inhibits the decomposition of polysaccharides and sucrose into glucose, slows down the absorption of sugar, and significantly reduces the concentration of blood sugar after meals. Due to its good pharmacokinetic characteristics and low toxicity, acarbose is the first-line drug for the treatment of type Ⅱ diabetes. It is widely used to reduce postprandial hyperglycemia and has broad application prospects. [0003] Due to the complex chemical structure of acarbose, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/045
Inventor 杨军杨秋艳程志鹏梁隆王利春沈鑫
Owner SHANGHAI RECOM BIOTECHNOLOGY CO LTD
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