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Solution for sealing and preserving magnetic nanoparticles

A technology of nano-magnetic particles and preservation solution, applied in the biological field, can solve the problems of reducing the sensitivity of reagents and the like

Active Publication Date: 2013-05-08
SUZHOU HAOOUBO BIOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the purpose of the present invention is to overcome the defects of the prior art and provide a closed preservation solution of magnetic nanoparticles, so as to avoid non-specific adsorption in the preservation process after the magnetic nanoparticles are coated with antigens or antibodies, resulting in loss of reagents. Defects with reduced sensitivity

Method used

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  • Solution for sealing and preserving magnetic nanoparticles
  • Solution for sealing and preserving magnetic nanoparticles
  • Solution for sealing and preserving magnetic nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Materials and Instruments:

[0027] Carcinoembryonic antigen monoclonal antibody-coated nano-magnetic particle chemiluminescent test reagent (CEA reagent), the suspension of nano-magnetic particles in the reagent is nano-magnetic particles coated with FITC (fluorescein isothiocyanate) antibody (that is, the suspension of CEA nano-magnetic particles); Maglia60 chemiluminescence immunoassay analyzer, commercially available.

[0028] Wherein, described CEA reagent comprises:

[0029] The CEA reagent includes a solution containing fluorescein-labeled CEA antibody (referred to as the first reagent) and a suspension of magnetic particles coated with fluorescein antibody (referred to as magnetic separation reagent), alkaline phosphatase (ALP)-labeled CEA antibody solution (referred to as the second reagent).

[0030] Described CEA reagent is prepared by the following method:

[0031] Preparation of the first reagent

[0032] (1) Materials and instruments: CEA antibody pres...

Embodiment 2

[0080] The CEA reagent used in this example is the same as that in Example 1, the difference is that the CEA nano-magnetic particles in the CEA reagent are sealed and stored with the following sealing preservation solution, and the sensitivity of the reagent is measured using a Maglia60 chemiluminescence immunoassay analyzer. In this embodiment, the detection steps of using the CEA reagent to detect the "0" concentration serum sample are also the same as in the first embodiment.

[0081] In this embodiment, the sealed preservation solution of CEA nano-magnetic particles is: water is used as a solvent, and each 1 L of the sealed preservation solution contains:

[0082] Tris 0.01mol pH8.0;

[0083] Bovine gamma globulin 2.5g;

[0084] Fish skin gel 5g;

[0085] Newborn bovine serum 5mL;

[0086] TWEEN-20 2.5g;

[0087] Neomycin sulfate 0.25g.

[0088] Sensitivity evaluation

[0089] Detect the "0" concentration serum sample, repeat the detection 20 times, calculate the mea...

Embodiment 3

[0102] The CEA reagent used in this example is the same as that in Example 1, the difference is that the CEA nano-magnetic particles in the CEA reagent are sealed and stored with the following sealing preservation solution, and the sensitivity of the reagent is measured using a Maglia60 chemiluminescence immunoassay analyzer. In this embodiment, the detection steps of using the CEA reagent to detect the "0" concentration serum sample are also the same as in the first embodiment.

[0103] In this embodiment, the sealed preservation solution of CEA nano-magnetic particles is: water is used as a solvent, and each 1 L of the sealed preservation solution contains:

[0104] Tris 0.01mol pH8.0;

[0105] Bovine gamma globulin 5g;

[0106] Fish skin gel 10g;

[0107] Newborn bovine serum 10mL;

[0108] TWEEN-20 5g;

[0109] Neomycin sulfate 0.5g.

[0110] Sensitivity evaluation

[0111] Detect the "0" concentration sample, repeat the detection 20 times, calculate the mean (M) and...

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Abstract

The invention provides a solution for sealing and preserving magnetic nanoparticles. The solution using water as solvent contains, calculated by 1L of the solution, Tris 0.01mol, bovine gamma-globulin 0.5 to 5g, fish skin gelatin 1 to 10g, new-born calf serum 1 to 10mL, emulsifying agent Tween 0.2 to 5g, and neomycin sulfate 0.01 to 0.5g. Compared with the prior art, the solution provided by the invention can effectively reduce nonspecific adsorption of the magnetic nanoparticles in immunoreaction when being used for sealing and preserving magnetic nanoparticles coated with antigens or antibodies, thereby improving the sensitivity of a reagent for determining chemiluminiscence of the magnetic nanoparticles.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a closed preservation solution of nano magnetic particles. Background technique [0002] Magnetic separation technology is based on nano- or particle-level magnetic particles as a carrier, using the specific affinity characteristics provided by proteins bound to the surface of magnetic particles, and under the directional control of a magnetic field, through affinity adsorption, cleaning, and desorption operations, It can further separate target molecules from complex biological systems, and has many advantages such as simple and convenient magnetic separation, high specificity and high sensitivity of affinity adsorption. The magnetic carrier used in magnetic separation technology should have the following characteristics: ① relatively small particle size, large specific surface area, and large adsorption capacity; ② stable physical and chemical properties, high mechanica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/533
Inventor 于大为程晓蕾
Owner SUZHOU HAOOUBO BIOPHARML
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