Preparation method of pure rice-koji added with Chinese medical herbs

A technology for Chinese medicinal materials and rice koji, applied in the field of pure-bred rice koji preparation, to achieve the effect of great development value and broad market prospect

Active Publication Date: 2013-05-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention combines Japanese pure strain rice koji preparation technology with my country's traditional medicinal koji concept, which has not been reported at home and abroad at present

Method used

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  • Preparation method of pure rice-koji added with Chinese medical herbs
  • Preparation method of pure rice-koji added with Chinese medical herbs
  • Preparation method of pure rice-koji added with Chinese medical herbs

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preparation example Construction

[0033] The preparation of the pure rice koji of embodiment 1 adding rhodiola medicinal koji

[0034] (1) Bacteria culture on inclined plane

[0035] Inoculate the preserved bacterial strain Aspergillus flavus QJ provided by Sichuan University Food Ecology Engineering and Biotechnology Laboratory to take freeze-dried tubes or sandy soil tubes or preserved slopes and inoculate them on PDA slant medium. The slant medium is potato juice 500ml / L, sucrose 20g / L, Agar 20g / L, pH natural), cultivated at 25°C for 7 days, selected slant colonies with uniform surface growth and thick spore layer, transplanted them into PDA medium again, and cultivated at 25°C for 7 days to obtain slant strains.

[0036] (2) Expanded cultivation of Aspergillus flavus QJ

[0037] Prepare PDA medium, pack 50ml / bottle into 250ml eggplant bottles, and sterilize at 121°C for 15 minutes. After the sterilization is completed, cool and tilt the slope to obtain the slope of the eggplant bottle. Take the above-men...

Embodiment 2

[0055] The preparation of the pure rice koji of embodiment 2 adding American ginseng medicinal koji

[0056] (1) Bacteria culture on inclined plane

[0057] Inoculate the preserved bacterial strain Aspergillus flavus QJ provided by Sichuan University Food Ecology Engineering and Biotechnology Laboratory to take freeze-dried tubes or sandy soil tubes or preserved slopes and inoculate them on PDA slant medium. The slant medium is potato juice 500ml / L, sucrose 20g / L, Agar 20g / L, pH natural, cultured at 30°C for 5 days, selected slant colonies with uniform surface growth and thick spore layer, transplanted into PDA medium again, and cultivated at 30°C for 5 days to obtain slant strains.

[0058] (2) Expanded cultivation of Aspergillus flavus QJ

[0059] Prepare PDA medium, pack 50ml / bottle into 250ml eggplant bottles, sterilize at a temperature of 118°C for 20 minutes, cool and tilt the slope to obtain the eggplant bottle slope after the sterilization is completed. Take the abov...

Embodiment 3

[0076] The preparation of the pure rice koji of embodiment 3 adding Astragalus medicinal koji

[0077] (1) Bacteria culture on inclined plane

[0078] Inoculate the preserved bacterial strain Aspergillus flavus QJ provided by Sichuan University Food Ecology Engineering and Biotechnology Laboratory to take freeze-dried tubes or sandy soil tubes or preserved slopes and inoculate them on PDA slant medium. The slant medium is potato juice 500ml / L, sucrose 20g / L, Agar 20g / L, pH natural, cultivated at 27°C for 6 days, selected slant colonies with uniform surface growth and thick spore layer, transplanted into PDA medium again, and cultivated at 27°C for 6 days to obtain slant strains.

[0079] (2) Expanded cultivation of Aspergillus flavus QJ

[0080] Prepare PDA medium, pack 50ml / bottle into 250ml eggplant bottles, and sterilize at 120°C for 18 minutes. After the sterilization is completed, cool and tilt the slope to obtain the slope of the eggplant bottle. Take the above-mention...

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Abstract

The invention discloses a preparation method of pure rice-koji added with Chinese medical herbs and application thereof. The preparation method is characterized by comprising the following steps of: inoculating aspergillus flavus QJ into a PDA (Potato Dextrose Agar) slant culture medium, culturing at 25-30 DEG C for 7-10 days, selecting slant colonies with uniform surface growth and thick spore layers, transplanting onto the PDA slant culture medium again, culturing at 25-30 DEG C for 5-7 days to obtain slant strains, and preserving at 4 DEG C for 15-30 days; carrying out amplification culture on the aspergillus flavus QJ, preparing spore suspension of the aspergillus flavus QJ, adding Chinese medical herbs into spread cooled steamed rice, and culturing medicinal koji for 3-4 days under the conditions that temperature is 35-40 DEG C and humidity is 90-95% so as to obtain the ure rice-koji added with the Chinese medical herbs, wherein the diastatic power is 979.348-1738.99 U/g dry koji; and the liquefaction force is 21.37-59.43 U/g dry koji. Compared with the rice-koji without Chinese medical herbs, the diastatic power of the medicinal koji is increased by 25.89-41.61%; the liquefaction force of the medicinal koji is increased by 31.97-56.122%; therefore, the pure rice-koji has great development value and wide market prospect.

Description

technical field [0001] The invention relates to a preparation method of pure rice koji added with Chinese medicinal materials, belonging to the field of brewing and fermentation. Background technique [0002] Medicinal koji is a saccharifying agent and leavening agent for brewing white wine and yellow rice wine. Traditional medicinal koji is generally made from rice flour or rice bran, mixed with Chinese herbal medicines, inoculated with mother koji, and made through billet making, powder coating, indoor cultivation, and room drying. Its finished song is white or light yellow, with uniform hyphae, no black, and special aroma (Zhang Wenxue. Overview of Chinese Wine Culture [M]. Beijing: China Light Industry Press, 2011). Medicinal koji has a long history. For example, a small song in Qionglai, Sichuan, is famous in the southwest. A total of 72 Chinese medicinal materials are added to the koji, and the medicinal materials used are crushed and dried into fine powder for use. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12G3/02C12J1/00A23L1/238C12R1/67A23L27/50
Inventor 张文学吴正云李丽赵盈盈罗颂罗芳
Owner SICHUAN UNIV
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