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Rapid in vitro breeding method of zucchini

A zucchini, in vitro technology, applied in the field of bioengineering, can solve the problems of browning of explants, low bud differentiation rate, long reproduction time, etc., and achieve the effect of accelerating the reproduction speed and shortening the physiological cycle

Inactive Publication Date: 2013-06-19
LANZHOU HONGQING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the propagation of zucchini is usually propagated by seeds, which takes a long time and has a low reproduction coefficient. Moreover, due to the limitation of the variety genotype, the bud differentiation rate is relatively low, while the pollution rate is relatively high, and the browning of explants occurs. In the process of using callus for cell culture, the cell biomass is not too high, which has limitations for the use of pumpkin tissue for cell culture and the production of secondary metabolites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Select zucchini seeds that are plump and of the same size, soak them in constant temperature water at 25°C for 7 hours, then disinfect the surface with 70% alcohol for 30 seconds on an ultra-clean bench, and place them in 0.1% HgCl 2 Disinfect respectively in the solution for 8min, shake continuously during the disinfection, then rinse 4 times with sterile water, put it into the aseptic seedling germination medium respectively under aseptic conditions after the seed disinfection, and cultivate the germination medium according to Add 8 g of agar and 20 g of sucrose to each 1 LMS, adjust the pH value to 5.8, incubator at 25°C, and incubate in the dark for 1 day;

[0018] Two, carry out 6h darkness to the germinated seeds, 20h light, and the illuminance is 80%, get its cotyledon, hypocotyl, radicle when aseptic seedling cotyledon just turns green and do explant induction callus; Get cotyledon Inoculate on the callus induction medium, remove the edge of the cotyledons fi...

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PUM

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Abstract

The invention discloses a rapid in vitro breeding method of zucchini, relates to a plant tissue culture technique, and belongs to the technical field of biological engineering. The invention has the beneficial effect that compared with the prior art, a tissue culture method can accelerate propagation speed, and under the culture conditions of the invention, the culture of aseptic seedlings, callus induction, bud differentiation and rooting culture greatly shorten a physiological cycle of zucchini; more importantly, the obtained callus tissue is suitable for further zucchini cell culture, and study on the secondary metabolites of zucchini cell; and the significance of the invention lays in that the method lays foundation for molecular breeding of zucchini, for the rapid propagation of zucchini variety and can obtain useful secondary metabolites of zucchini.

Description

technical field [0001] The invention relates to a plant tissue culture technology, which belongs to the technical field of bioengineering, in particular to a method for in vitro rapid propagation of zucchini. Background technique [0002] Zucchini (Cucurbita pepo L.), aliases: watermelon, pumpkin, etc., is an annual vine herb of Cucurbitaceae Cucurbita. It is native to southern North America and is now widely cultivated. The zucchini cultivated early in spring in my country is the earliest vegetable to be marketed in early spring. It has made a positive contribution to the transition of vegetables in spring, and it is also a main variety for vegetable farmers to obtain high-efficiency cultivation. In recent years, it has been found that zucchini has great value in health care, nutrition, and medical treatment; its rich water has the effects of clearing away heat and diuresis, relieving restlessness and quenching thirst, moisturizing the lungs and relieving cough, reducing s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 房世平
Owner LANZHOU HONGQING TECH
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