Reagent device and method for detecting anti-myeloperoxidase antibody

Abstract

The invention provides a reagent device and a method used for detecting anti-myeloperoxidase antibodies. The reagent device has a long strip shape, and has a base body comprising 8 hole positions and a handle positioned on one end of the base body. From the end proximal to the handle, the 8 hole positions are sequentially a sample hole, an auxiliary agent hole, an enzyme conjugate hole, a substrate hole, a termination liquid hole, a dilution liquid hole, a reaction hole, and a dilution hole. According to the invention, based on a principle of enzyme-linked immunoassay, anti-myeloperoxidase antibody is detected by using the reagent device. The method is an independent single-person analysis and detection method. The device can be used in cooperation with a corresponding specific analysis instrument. During a detection process, detection reagents or samples are injected by using a full-automatic precise dosing device. The device and the method have the advantages of automatic operation, precise dosing, high detection result accuracy, high detection result precision, and wide application prospect.

Description

technical field [0001] The application of the present invention relates to a reagent device and method for detecting anti-myeloperoxidase antibodies, belonging to the technical field of clinical immunology detection. Background technique [0002] Vasculitis is a group of inflammatory diseases with inflammation and necrosis of blood vessels as the main pathological changes. The clinical manifestations vary according to the type, size, location and pathological characteristics of the blood vessels involved. It often involves multiple systems of the whole body (such as skin, kidney, lung, nervous system, etc.), causing dysfunction of multiple systems and organs, but it can also be limited to a certain organ. [0003] Myeloperoxidase (MPO) is the main target antigen of anti-neutrophil cytoplasmic antibody (ANCA), and it is an important substance for neutrophils to kill and phagocytize microorganisms. Cytoplasmic components of neutrophils and monocytes. Anti-MPO antibodies can ...

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Problems solved by technology

[0007] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results;

[0008] (2) The operation is relatively complicated and requires expensive fluorescence microscopes, which are difficult to popularize in many primary hospitals and are not suitable for laboratories with a large number of specimens;

[0009] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination;

[0010] (4) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient;

[0011] (5) Only qualitative detection can be carried out, but quantitative determination cannot be carried out;

[0015] (1) Use 12×8 type, 6×8 type, 8×12 type or whole plate type 96-well special microwell plate as antigen coating equipment and reaction Containers can only be divided into 12 batches, 6 batches, 8 batches or the whole board for one-time use, and independent and single-person testing cannot be carried out;

[0016] (2) There are many kinds of reagents used in quantitative determination, and each detection reagent must be contained in a reagent bottle, and each time a reagent is used, it needs to be replaced The liquid suction nozzle is used to fill the microwells of the microwell plate, not only the variety of reagent bottles, but also the operation of adding reagents is extremely cumbersome;

[0017](3) There is a lack of corresponding labeling of the test information, and the production batch number and expiration date information of the test reagent can only be known or known by checking the label on the outer packaging box of the test kit, Moreover, the known information is not controlled during the detection process and has great randomness;

[0018](4) The detection reagent is in an open space during the detection process, which may easily cause cross-contamination among various reagents and affect the accuracy of the detection result;

[0019](5) The detection process is mostly manual operation, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur. Poor accuracy and precision;

[0020](6) The quantity configuration and use of the complete set of reagents for the test items are the number of items × 48 / 96 servings. If 10 items need to be tested, the reagent’s The number of configuration and use must be 10×48 / 96. If only one sample needs to be tested for 10 different items, it is also necessary to configure 10×48 / 96 reagents, which is not economical and reasonable.

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