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Devices and methods for controlling actin filaments growth and organization using micropatterned nucleation sites

An actin filament and actin technology, applied in chemical instruments and methods, measuring devices, compound screening, etc., can solve the problem of no orientation of actin filaments

Inactive Publication Date: 2013-07-10
原子能及替代能源委员会 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, actin filaments growing from nucleation sites are not oriented and must be driven externally by beads manipulated by magnetic or optical traps
[0011] None of these methods allow to provide self-assembly of ordered actin filament networks with reproducible and controlled geometries

Method used

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  • Devices and methods for controlling actin filaments growth and organization using micropatterned nucleation sites
  • Devices and methods for controlling actin filaments growth and organization using micropatterned nucleation sites
  • Devices and methods for controlling actin filaments growth and organization using micropatterned nucleation sites

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Embodiment

[0122]Actin filaments constitute one of the main components of the cytoskeleton. Specific actin cytoskeletal structures, such as branched networks or parallel actin filament bundles, drive well-defined cellular functions. Biochemical reconstruction of actin network assemblies in bulk solution or from purified proteins using biomimetic devices has emerged as a powerful tool to circumvent cellular complexity and decipher the molecular mechanisms governing actin assembly. These methods highlight how actin-binding proteins affect the growth and interaction of actin filaments in dynamic networks. However, how geometric boundaries such as those encountered in cells affect the dynamic formation of highly ordered actin structures remains largely unexplored.

[0123] Here, the inventors demonstrate that nucleation geometry itself may be the primary determinant of actin network architecture. They developed a micropatterning method that allows spatial control of actin nucleation sites ...

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Abstract

The present invention concerns devices and methods for controlling actin filaments growth and organization with micro patterned nucleation sites, their uses for studying actin network formation, for screening of drugs or for preparing complex structures.

Description

technical field [0001] The present invention relates to devices and methods for controlling the organization of the actin filament network and thereby for studying the organization of the actin filament network, for screening compounds or For the fabrication of actin-based assemblies, especially using actin networks as templates for other nanoengineering processes. Background technique [0002] Actin filaments constitute one of the main components of the cytoskeleton. Actin filaments are polymers that spontaneously self-assemble into cells from actin monomers. They form complex intracellular structures that provide mechanical support for regulating cell shape. Assembled in bundles as filopodia or as stress fibers, they play key roles during cell morphogenesis, adhesion and motility. The emergence of such tracts has been widely associated with specific actin regulators in vivo. Such dynamic regulation has also been highlighted by biochemical reconstitution of actin networ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6887G01N2333/4712G01N2500/20C07K14/435
Inventor 玛纽尔·特里劳伦特·布兰可因拉贾·帕特尔斯基
Owner 原子能及替代能源委员会
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