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Application of SOAR1 protein and encoding gene thereof to regulation of plant adversity stress resistance

A technology that encodes genes and proteins, applied in the biological field, to achieve the effect of improving stress resistance and tolerance

Inactive Publication Date: 2014-12-03
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only a few PPR proteins have been found in Arabidopsis thaliana to participate in the stress resistance process, such as PPR40, ABO5, SLG1, PGN and AHG11, etc. This is only the tip of the iceberg in the huge terrestrial PPR protein family, and more regulation plants The PPR protein that resists adversity stress remains to be discovered

Method used

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  • Application of SOAR1 protein and encoding gene thereof to regulation of plant adversity stress resistance
  • Application of SOAR1 protein and encoding gene thereof to regulation of plant adversity stress resistance
  • Application of SOAR1 protein and encoding gene thereof to regulation of plant adversity stress resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the acquisition and identification of SOAR1 transgenic plants

[0055] The SOAR1 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown as sequence 1 in the sequence listing. Sequence 1 consists of 2216 nucleotides, of which the 653rd Position -763 is an intron sequence; the cDNA sequence of the SOAR1 gene is shown in sequence 2 in the sequence table, sequence 2 is composed of 2105 nucleotides, and the 89th-1897th position is the coding sequence (ORF); sequence 1 Both Sequence 2 and Sequence 2 encode the protein shown in Sequence 3 in the sequence listing, and Sequence 3 consists of 602 amino acid residues.

[0056] According to bioinformatics prediction, SOAR1 is a member of the PPR protein family.

[0057] exist http: / / www.ebi.ac.uk / Tools / InterProScan / index.htmla The SOAR1 protein was predicted in the database, and it was found that the SOAR1 protein was composed of an N-terminal signal ...

Embodiment 2

[0136] Example 2, SOAR1 transgenic plant salt stress analysis test

[0137] Salt stress, as an important abiotic stress, refers to the stress effect of high osmotic potential on plant growth in a high-salt environment. The plant's resistance to salt stress mainly relies on the physiological process of cells synthesizing some substances that are not harmful to the protoplast to adjust the cell osmotic potential, thereby resisting the damage of salt stress.

[0138] 1. Effect of NaCl on seed germination of SOAR1 genetic materials

[0139] The single-copy inserted T3 generation homozygous SOAR1 transgene obtained from Arabidopsis wild type (Col-0 ecotype), two T-DNA insertion mutants soar1-2 and soar1-3 with reduced SOAR1 gene expression, and Example 1 The strains OE1, OE3 and OE6, and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as experimental materials. The seeds of each experimental material were sown on MS medium con...

Embodiment 3

[0145] Embodiment 3, SOAR1 transgenic plant drought resistance analysis test

[0146] In this example, mannitol was used to simulate the adversity stress of drought on plants.

[0147] 1. Effect of mannitol on seed germination of SOAR1 genetic material

[0148] The single-copy inserted T3 generation homozygous SOAR1 transgene obtained from Arabidopsis wild type (Col-0 ecotype), two T-DNA insertion mutants soar1-2 and soar1-3 with reduced SOAR1 gene expression, and Example 1 The strains OE1, OE3 and OE6, and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as experimental materials. The seeds of each experimental material were sown on MS medium containing different concentrations of mannitol (0mM and 300mM) (80-100 seeds were sown for each experimental material). After low-temperature stratification at 4°C for 3 days, they were transferred to a light incubator. Record the germination number of seeds between 24h and 120h af...

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Abstract

The invention discloses an application of an SOAR1 protein and an encoding gene thereof to the regulation of plant adversity stress resistance. The application provided by the invention concretely relates to an application of a protein composed of an amino acid sequence shown in a sequence 3 in a sequence table to the following a1 or a2; a1: regulating the plant stress resistance; and a2: breeding a plant variety with improved stress resistance. Experiments prove that the tolerance of an SOAR1 transgenetic plant obtained by the invention to various abiotic adversity stresses such as salt, drought, low temperature and osmosis is improved as compared with that of a non-transgenic reference plant. The SOAR1 protein and the encoding gene thereof have important significance on the aspects of a plant adversity stress resistance molecular mechanism and molecular breeding research and an important practical value and wide market prospect on the aspect of improving the adversity stress resistance and tolerance of crops to climate and weather disasters and poor soil environments.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a SOAR1 protein and its coding gene in regulating plant resistance to adversity stress. Background technique [0002] Terrestrial plants may be affected by adversity stresses throughout their growth and development stages, among which abiotic stresses such as drought, salinity, low temperature (chilling injury and freezing injury) are important factors that strongly restrict plant growth and crop yield. Plants have evolved a series of strategies to resist, tolerate and avoid adversity stress for survival. The research on plant stress resistance has always been a hot spot in the field of botany. Traditional breeding techniques are difficult to improve stress tolerance traits, but with the development of molecular biology technology and in-depth research on the molecular mechanism of plant stress resistance, significant progress has been made in genetic engineering of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/84A01H5/00
Inventor 王小芳姜上川梅超张大鹏
Owner TSINGHUA UNIV