Preparation method and application thereof for cell-biological bracket compound based on biological print technology

Abstract

The present invention provides a method for preparing a cell-biological scaffold complex based on bioprinting technology and its application. The cell-biological complex is prepared from fibrin extracted from the patient's own blood through inkjet printing technology. The surface and the inside are coated with one or several nutritional factors and bone marrow mesenchymal stem cells derived from the body. The present invention utilizes the bioprinting technology to design the appearance shape and the coating state model of cells and nutritional factors according to the needs and actual conditions, and accurately print to obtain the cell-biological scaffold complex, and the fibrin and bone marrow mesenchymal stem cells are all derived from Autologous, to avoid immune rejection and other problems, and the nutrient factors are planted inside the scaffold, slowly released with the degradation of the scaffold material, and act on the spinal cord injury site for a long time to promote nerve regeneration and functional restoration of the injured site.

Description

technical field [0001] The invention relates to the construction of a cell-bio-scaffold compound for treating spinal cord injury, in particular to a preparation method and application of the cell-bio-scaffold compound based on bioprinting technology. Background technique [0002] Spinal cord injury is a serious traumatic disease of the central nervous system, which has the characteristics of high consumption and high disability rate, and brings huge damage and heavy burden to the society. Difficulty in nerve regeneration after spinal cord injury due to interruption of spinal cord continuity, neuronal loss, lack of neurotrophic factors, glial scarring, and cavitation. Traditional treatment methods such as drugs and surgery cannot solve the problem of regeneration, while the source of allogeneic acellular scaffolds is limited and there is a large immune rejection. In recent years, basic research, especially the development of nerve tissue engineering technology, has made grea...

AI Technical Summary

Problems solved by technology

Although this method has achieved some great success in the construction of simple cavity tissues and organs, it has many shortcomings, especially in the construction of tissues and organs with complex internal structures. In this method, cells cannot be precisely planted into The inside of the scaffold is only randomly adsorbed on the surface. Cell activity factors can only affect the growth and differentiation of cells on the surface of the scaffold, but it is difficult to control the activity of cells inside the scaffold. In view of the limitations of traditional tissue engineering methods, looking for more scientific tissue engineering technology is imminent