Method for culturing hericium erinaceus by persimmon sawdust

Abstract

The invention relates to a method for culturing hericium erinaceus by utilizing persimmon sawdust of forestry wastes as main culturing materials, adopts screening and breeding of hericium erinaceus strains suitable for decomposition of persimmon sawdust and belongs to the technical field of utilization of agriculture and forestry wastes and culture of edible and medical fungi. The method includes steps of A, screening the hericium erinaceus strains with persimmon sawdust decomposition advantages; B, expanding propagation of the strains; C, culturing the hericium erinaceus. Since the persimmon sawdust powder is used as a unique carbon source capable of guiding to screen strains better for effectively utilizing the sawdust in the step of screening in ultraviolet mutagenesis breeding of the hericium erinaceus, screening and breeding efficiency of the strains with decomposition advantages are improved; in the process of liquid strain culture, the persimmon sawdust is used as a special base, and by corresponding enzymes generated due to induction, adaptation time of the strains on a culture medium taking the persimmon sawdust as main materials can be shortened, and growth speed of the hericium erinaceus is improved. Accordingly, the method provides a technical scheme that persimmon sawdust of agricultural and forestry wastes can be effectively utilized by screening and breeding the hericium erinaceus beneficial for utilizing the persimmon sawdust.

Description

technical field

[0001] The invention relates to a method for cultivating and cultivating Hericium erinaceus using forestry waste persimmon sawdust as the main material, including the breeding process of Hericium erinaceus strains suitable for decomposing persimmon sawdust, and belongs to the technical field of utilization of agricultural and forestry residues and cultivation of edible and medicinal fungi . Background technique

[0002] There are more than 200 varieties of persimmon trees, which are widely distributed in China and are planted in both north and south. Persimmon has high nutritional value and is a popular fruit. It is often made into dried fruit and exported to Southeast Asia. Therefore, as a high economic additional fruit tree, persimmon plays an important role in the adjustment of agricultural economic structure in some areas. Because persimmon trees have a large amount of flowers and fruits and consume a lot of nutrients, they are prone to the phenomenon of...

Examples

Embodiment Construction

[0033] The Hericium erinaceus strain used in the technical solution of the present invention is quoted from the Institute of Edible Fungi of the Shanghai Academy of Agricultural Sciences.

[0034] Hericium erinaceus belongs to Basidiomycota, Agaricomycetes, Russulales, and Hericiaceae. It is a famous edible and medicinal fungus.

[0035] A. Screen Hericium ericaceus strains with the advantage of decomposing persimmon sawdust:

[0036] A-1, ultraviolet mutagenesis of Hericium erinaceus:

[0037] 1) Mycelium preparation: The medium formula is 20% peeled potato, 2% bran, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O1g / L, pH 5.0, agar 0.4%. The culture condition was 160r / min, and the temperature was 25°C in a constant temperature shaker for 7 days.

[0038] 2) Protoplast preparation:

[0039]2-1) Place the cultured mycelium in a centrifuge tube and centrifuge at 6000r / min for 10min, remove the supernatant, wash three times with 109g / L mannitol osmotic pressure stabilizer, add 1mL for ever...

technical field

[0001] The invention relates to a method for cultivating and cultivating Hericium erinaceus using forestry waste persimmon sawdust as the main material, including the breeding process of Hericium erinaceus strains suitable for decomposing persimmon sawdust, and belongs to the technical field of utilization of agricultural and forestry residues and cultivation of edible and medicinal fungi . Background technique

[0002] There are more than 200 varieties of persimmon trees, which are widely distributed in China and are planted in both north and south. Persimmon has high nutritional value and is a popular fruit. It is often made into dried fruit and exported to Southeast Asia. Therefore, as a high economic additional fruit tree, persimmon plays an important role in the adjustment of agricultural economic structure in some areas. Because persimmon trees have a large amount of flowers and fruits and consume a lot of nutrients, they are prone to the phenomenon of...

Examples

Embodiment Construction

[0033] The Hericium erinaceus strain used in the technical solution of the present invention is quoted from the Institute of Edible Fungi of the Shanghai Academy of Agricultural Sciences.

[0034] Hericium erinaceus belongs to Basidiomycota, Agaricomycetes, Russulales, and Hericiaceae. It is a famous edible and medicinal fungus.

[0035] A. Screen Hericium ericaceus strains with the advantage of decomposing persimmon sawdust:

[0036] A-1, ultraviolet mutagenesis of Hericium erinaceus:

[0037] 1) Mycelium preparation: The medium formula is 20% peeled potato, 2% bran, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O1g / L, pH 5.0, agar 0.4%. The culture condition was 160r / min, and the temperature was 25°C in a constant temperature shaker for 7 days.

[0038] 2) Protoplast preparation:

[0039]2-1) Place the cultured mycelium in a centrifuge tube and centrifuge at 6000r / min for 10min, remove the supernatant, wash three times with 109g / L mannitol osmotic pressure stabilizer, add 1mL for ever...

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