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Method for preparing compound enzyme and probiotic preparation for feed through biotransformation of corn stalks and soybean meals

A technology of probiotics preparation and corn stalk, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problem of difficult digestion of monogastric animals, and achieve the effect of low-cost and high-efficiency biotransformation and strong enzyme production capacity.

Active Publication Date: 2014-09-24
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corn stalks, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0027] 2) Fully mix 20kg of the starter in step 1) with 600kg of corn stalks, 300kg of soybean cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, and ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0028] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 74.52IU / g; total cellulase activity reaches 5.65IU / g; beta-glucosidase activity reaches 5.65IU / g; reached 13.24IU / g; endoglucanase activity reached 55.22IU / g; xylanase activity reached 68.38IU / g; beta-xylosidase activity reached 3.89I...

Embodiment 2

[0030] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corn stalks, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0031] 2) Fully mix 20kg of the starter in step 1) with 600kg of corn stalks, 300kg of soybean cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, and ferment at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0032] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 73.63IU / g; total cellulase activity reaches 5.71IU / g; beta-glucosidase activity reaches 5.71IU / g; reached 13.18IU / g; endoglucanase activity reached 55.26IU / g; xylanase activity reached 67.98IU / g; beta-xylosidase activity reached 3.91I...

Embodiment 3

[0034] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corn stalks, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 40°C for 36 hours to form a starter;

[0035] 2) Fully mix 10kg of starter in step 1) with 600kg of corn stalks, 300kg of soybean meal, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and ferment at 35°C for 5 Day, get the compound enzyme and probiotic preparation for feed.

[0036] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 68.55IU / g; total cellulase activity reaches 6.12IU / g; beta-glucosidase activity reaches 68.55IU / g; reached 11.84IU / g; endoglucanase activity reached 53.29IU / g; xylanase activity reached 65.54IU / g; beta-xylosidase activity reached 4.23IU / g; polygal...

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed through the biotransformation of corn stalks and soybean meals. The method comprises the following steps of: thoroughly mixing cooked Pu'er tea, first corn stalks, first soybean meals, first wheat bran, first apple pomace and first water which are subjected to pile fermentation, and carrying out acclimation culture so as to form a leavening agent; and thoroughly mixing the leavening agent, second corn stalks, second soybean meals, second wheat bran, second apple pomace and second water, and carrying out fermentation, thereby obtaining the compound enzyme and probiotic preparation for feed. According to the method, the compound enzyme and probiotic preparation with better various performance indexes is prepared through fermenting the corn stalks and the soybean meals by using food-grade safe flora with stable microbial states, so that the low-cost and high-efficiency biotransformation of the corn stalks and the soybean meals is realized. Meanwhile, the method disclosed by the invention has the advantages that microbes grow easily, the enzyme activity is high, the fermentation process is extensive, strict aseptic conditions are not required, and the industrial large-scale production is facilitated.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting corn stalks and soybean cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 阮晖项云徐志钱春芳陈功诸文颖纪晓燚吴渊杜姗姗杨璐
Owner JINHUA ACAD OF AGRI SCI
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