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Bletilla seed tissue culture propagation method

A technology of seed group and Bletilla striata, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low natural germination rate of seeds, unable to meet market needs, scarce wild resources, etc. The effect of shortening production time and stabilizing hereditary traits

Inactive Publication Date: 2013-09-25
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The natural germination rate of Bletilla striata seeds is extremely low, reproduction is difficult, and wild resources are scarce. Due to the large demand, wild resources are already on the verge of depletion. They have been listed in the "Convention on International Trade in Endangered Species of Animals and Plants" for protection. Therefore, only artificial breeding is adopted. Most of the current conventional methods are based on division, that is, the tubers are cut into small pieces for planting, and the reproduction coefficient is low, which can no longer meet the needs of the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare the medium first:

[0023] Protocorm induction culture: ?MS+6-BA0.9mg / L+NAA0.11mg / L+sucrose 30g / L+agar 5g / L; pH=5.8.

[0024] Protocorm proliferation culture: ?MS+6-BA1.1mg / L+NAA0.11mg / L+sucrose 30g / L+agar 5g / L; pH=5.8.

[0025] Cluster bud induction culture: BM+BA4+IAA1+carrageenan 5g / L+sucrose 30g / L; pH=5.8.

[0026] Bud proliferation culture: BM++IAA0.3+carrageenan 5g / L+sucrose 30g / L; pH=5.8.

[0027] Rooting culture: BM+IAA1+carrageenan 5g / L+sucrose 30g / L; pH=5.6.

[0028] Select robust wild Bletilla striata capsules, wash them under running water, soak them in 1% detergent solution for 5 minutes, then gently scrub them with a brush, and wash them thoroughly with sterile water; place the seeds on an ultra-clean bench After sterilizing in 75% alcohol for 30 seconds, use 0.1% HgCl 2 Soak in aqueous solution for 30 minutes, then rinse with sterile water for 5-6 times, then place it on sterile filter paper to absorb water, peel off from the middle of the fru...

Embodiment 2

[0030] Select robust wild Bletilla striata capsules, wash them under running water, soak them in 1% detergent solution for 5 minutes, then gently scrub them with a brush, and wash them thoroughly with sterile water; place the seeds on an ultra-clean bench After sterilizing in 75% alcohol for 30 seconds, use 0.1% HgCl 2Soak in aqueous solution for 30 minutes, then rinse with sterile water for 5-6 times, then place it on sterile filter paper to absorb water, peel off from the middle of the fruit, and take out the seeds; quickly inoculate the seeds on the protocorm induction medium, and inoculate at night 20°C, day temperature 22°C, relative humidity 69%, carry out seed induction culture under dark box conditions, after 6-8 days, the seeds start to germinate and expand; adjust the cultivation environment at this time, at night temperature 21°C, day temperature 23°C, relative humidity 71%, the light intensity is 2000lx, the daily light start time is 7:00 in the morning, and the da...

Embodiment 3

[0032] Select robust wild Bletilla striata capsules, wash them under running water, soak them in 1% detergent solution for 5 minutes, then gently scrub them with a brush, and wash them thoroughly with sterile water; place the seeds on an ultra-clean bench After sterilizing in 75% alcohol for 30 seconds, use 0.1% HgCl 2 Soak in aqueous solution for 30 minutes, then rinse with sterile water for 5-6 times, then place it on sterile filter paper to absorb water, peel off from the middle of the fruit, and take out the seeds; quickly inoculate the seeds on the protocorm induction medium, and inoculate at night 20°C, daily temperature 22°C, relative humidity 70%, carry out seed induction cultivation under dark box conditions, after 6-8 days, the seeds start to germinate and expand; adjust the cultivation environment at this time, at night temperature 21°C, day temperature 23°C, relative humidity 72%, the light intensity is 2000lx, the daily light start time is 7:00 in the morning, and...

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PUM

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Abstract

The invention discloses a bletilla seed tissue culture propagation method which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following six steps of seed introduction and sterilization, induction culture of protocorm, enrichment culture of protocorm, induction culture of multiple shoots, enrichment culture of multiple shoots, and rooting culture. The method has the beneficial effects that seedlings generated by adopting seed tissue culture is low in pollution rate, high in differentiation rate, stable in hereditary characters, and high in active substance content. According to the method disclosed by the invention, the tissue culture propagation period of bletilla is also greatly shortened, and the tissue culture period from seeding to rooting is less than four months, so that the production time is shortened, and the production cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, in particular to a method for tissue culture and propagation of bletilla striata seeds. Background technique [0002] Bletilla striata is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae, also known as white root, ground screw, white chicken baby, horn seven, purple orchid, knife-edge medicine, Lianjicao, etc. The tubers are used for medicine, which can restrain bleeding, reduce swelling and promote granulation; treat diseases such as tuberculosis and hemoptysis, bronchiectasis hemoptysis, gastric ulcer, hematemesis, hematuria and hematochezia. External use to treat traumatic bleeding, burns, chapped hands and feet embolism. It has huge commercial application value in biomedicine, health food, textile printing and dyeing, special coatings and daily chemical industry. With the development and utilization of bletilla striata food an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨再江刘湘凤顾君龚成珍祝利
Owner 重庆市秀山红星中药材开发有限公司
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