Method for preparing composite enzyme/probiotic preparation for feed by bioconverting soybean stem and leaf and tea seed cake pulp

Abstract

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting soybean stem and leaf and tea seed cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first soybean stem and leaf, first tea seed cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second soybean stem and leaf, second tea seed cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the soybean stem and leaf and tea seed cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the soybean stem and leaf and tea seed cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

Description

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting soybean stems and leaves and tea seed cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique

[0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed.

[0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose,...

Examples

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water at 35 After 48 hours of domestication and cultivation at ℃, a starter culture is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40 ℃ for 4 days to obtain a compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 52.15IU / g; total cellulase activity reaches 5.88IU / g; beta-glucosidase activity reaches 5.88IU / g; reached 14.37IU / g; endoglucanase activity rea...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of domestication and cultivation at ℃, a starter culture is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40 ℃ for 4 days to obtain a compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 51.65IU / g; total cellulase activity reaches 5.92IU / g; beta-glucosidase activity reaches 5.92IU / g; reached 14.52IU / g; endoglucanase activity reached...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of domestication and cultivation at ℃, a starter culture is formed;

[0036] 2) Mix 10kg of the starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm. Naturally ventilated at 35°C Ferment for 5 days to obtain the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 53.86IU / g; total cellulase activity reaches 5.65IU / g; beta-glucosidase activity reaches 53.86IU / g; reached 14.35IU / g; endoglucanase activity...

technical field

[0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting soybean stems and leaves and tea seed cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique

[0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed.

[0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose,...

Examples

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water at 35 After 48 hours of domestication and cultivation at ℃, a starter culture is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40 ℃ for 4 days to obtain a compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 52.15IU / g; total cellulase activity reaches 5.88IU / g; beta-glucosidase activity reaches 5.88IU / g; reached 14.37IU / g; endoglucanase activity rea...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of domestication and cultivation at ℃, a starter culture is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40 ℃ for 4 days to obtain a compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 51.65IU / g; total cellulase activity reaches 5.92IU / g; beta-glucosidase activity reaches 5.92IU / g; reached 14.52IU / g; endoglucanase activity reached...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of soybean stems and leaves, 5kg of tea seed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of domestication and cultivation at ℃, a starter culture is formed;

[0036] 2) Mix 10kg of the starter in step 1) with 600kg of soybean stems and leaves, 300kg of tea seed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm. Naturally ventilated at 35°C Ferment for 5 days to obtain the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 53.86IU / g; total cellulase activity reaches 5.65IU / g; beta-glucosidase activity reaches 53.86IU / g; reached 14.35IU / g; endoglucanase activity...