A kind of saline-alkali land improvement agent of compound microbial bacteria and its preparation method and application
A technology of composite microbial bacteria and microbial bacterial agents, applied in the fields of application, fertilizer mixture, chemical instruments and methods, etc., can solve problems such as harmful anions and cations, low organic matter content in saline-alkali soil, affecting crop rooting and promoting seedlings, etc., to achieve The effect of promoting rice rooting, rice seedling robustness, and increasing number
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Embodiment 1
[0047] The invention relates to a saline-alkali improvement agent of compound microbial bacteria, and the improvement agent includes microorganisms, organic liquid and matrix.
[0048] Further, the microorganisms include Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium (Bacillus megaterium), and the weight and number ratio of the Bacillus subtilis and Bacillus megaterium is:
[0049] Bacillus subtilis 60~80
[0050] Bacillus megaterium 20-40;
[0051] Optimum parts by weight ratio is:
[0052] Bacillus subtilis 70
[0053] Bacillus megaterium 30.
[0054] Further, the Bacillus subtilis is a mixture of the Bacillus subtilis CGMCC7296, Bacillus subtilis CGMCC7297 and Bacillus subtilis CGMCC7298; the Bacillus megaterium is CGMCC7337.
[0055]The organic liquid described in this embodiment includes the protease, lipase, α-amylase and amino acid produced by the fermentation of the microbial agent.
[0056] The matrix described in the present embodiment comprises c...
Embodiment 2
[0060] A kind of preparation method of composite microbial element saline-alkali land improving agent, the manufacturing process steps of described improving agent are as follows:
[0061] The compound microbial bacteriocin saline-alkali soil improver is obtained by fermentation of 3 strains of Bacillus subtilis and 1 strain of Bacillus megaterium;
[0062] The three Bacillus subtilis strains are Bacillus subtilis CGMCC7296, Bacillus subtilis CGMCC7297, and Bacillus subtilis CGMCC7298; the Bacillus megaterium is CGMCC7337.
[0063] 1) Strain preparation
[0064] The 3 strains of Bacillus aspartate and 1 strain of Bacillus megaterium were separately activated on the slant of ordinary broth to obtain the activated strains; the activated strains were respectively transferred into flat bottles, 28~ Cultivate at 30°C for 24 hours, and prepare No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, and No. 4 seed suspension with 0.1 peptone sterile aqueous solution;
...
Embodiment 3
[0084] This embodiment is an improvement on the basis of Embodiment 2, and the same parts as Embodiment 2 will not be repeated.
[0085] The preparation method of the composite microbial element saline-alkali soil improver of the present embodiment, the manufacturing process steps of described improver are as follows:
[0086] 2) Cultivation of seed tanks
[0087] The formula of culture medium in the A seed tank and the ratio of parts by weight thereof are:
[0088]
[0089]
[0090] The temperature of the A seed tank is 34°C, and the pH of the cultivation is 7.5;
[0091] The formula of culture medium in the B seed tank and the ratio of parts by weight thereof are:
[0092]
[0093] 3) Fermentation tank fermentation
[0094] The culture medium formula of fermentor and its weight and number ratio are:
[0095]
[0096]
[0097] 4) Adsorbed on the matrix
[0098] Fully stir the fermented bacterial liquid and the substrate evenly, according to 20-30L / m 3 Ads...
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