A kind of saline-alkali land improvement agent of compound microbial bacteria and its preparation method and application

A technology of composite microbial bacteria and microbial bacterial agents, applied in the fields of application, fertilizer mixture, chemical instruments and methods, etc., can solve problems such as harmful anions and cations, low organic matter content in saline-alkali soil, affecting crop rooting and promoting seedlings, etc., to achieve The effect of promoting rice rooting, rice seedling robustness, and increasing number

Active Publication Date: 2016-04-20
TSINGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkaline soil refers to the soil containing carbonate or heavy phosphate, the pH value is high, the soil is alkaline, the organic matter content of saline-alkali soil is small, the soil fertility is low, the physical and chemical properties are poor, and there are many anions and cations harmful to crops, which seriously affect Crop rooting promotes seedlings, resulting in low grain yield

Method used

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  • A kind of saline-alkali land improvement agent of compound microbial bacteria and its preparation method and application
  • A kind of saline-alkali land improvement agent of compound microbial bacteria and its preparation method and application
  • A kind of saline-alkali land improvement agent of compound microbial bacteria and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The invention relates to a saline-alkali improvement agent of compound microbial bacteria, and the improvement agent includes microorganisms, organic liquid and matrix.

[0048] Further, the microorganisms include Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium (Bacillus megaterium), and the weight and number ratio of the Bacillus subtilis and Bacillus megaterium is:

[0049] Bacillus subtilis 60~80

[0050] Bacillus megaterium 20-40;

[0051] Optimum parts by weight ratio is:

[0052] Bacillus subtilis 70

[0053] Bacillus megaterium 30.

[0054] Further, the Bacillus subtilis is a mixture of the Bacillus subtilis CGMCC7296, Bacillus subtilis CGMCC7297 and Bacillus subtilis CGMCC7298; the Bacillus megaterium is CGMCC7337.

[0055]The organic liquid described in this embodiment includes the protease, lipase, α-amylase and amino acid produced by the fermentation of the microbial agent.

[0056] The matrix described in the present embodiment comprises c...

Embodiment 2

[0060] A kind of preparation method of composite microbial element saline-alkali land improving agent, the manufacturing process steps of described improving agent are as follows:

[0061] The compound microbial bacteriocin saline-alkali soil improver is obtained by fermentation of 3 strains of Bacillus subtilis and 1 strain of Bacillus megaterium;

[0062] The three Bacillus subtilis strains are Bacillus subtilis CGMCC7296, Bacillus subtilis CGMCC7297, and Bacillus subtilis CGMCC7298; the Bacillus megaterium is CGMCC7337.

[0063] 1) Strain preparation

[0064] The 3 strains of Bacillus aspartate and 1 strain of Bacillus megaterium were separately activated on the slant of ordinary broth to obtain the activated strains; the activated strains were respectively transferred into flat bottles, 28~ Cultivate at 30°C for 24 hours, and prepare No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, and No. 4 seed suspension with 0.1 peptone sterile aqueous solution;

...

Embodiment 3

[0084] This embodiment is an improvement on the basis of Embodiment 2, and the same parts as Embodiment 2 will not be repeated.

[0085] The preparation method of the composite microbial element saline-alkali soil improver of the present embodiment, the manufacturing process steps of described improver are as follows:

[0086] 2) Cultivation of seed tanks

[0087] The formula of culture medium in the A seed tank and the ratio of parts by weight thereof are:

[0088]

[0089]

[0090] The temperature of the A seed tank is 34°C, and the pH of the cultivation is 7.5;

[0091] The formula of culture medium in the B seed tank and the ratio of parts by weight thereof are:

[0092]

[0093] 3) Fermentation tank fermentation

[0094] The culture medium formula of fermentor and its weight and number ratio are:

[0095]

[0096]

[0097] 4) Adsorbed on the matrix

[0098] Fully stir the fermented bacterial liquid and the substrate evenly, according to 20-30L / m 3 Ads...

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Abstract

The invention relates to a composite microbiological bacteriocin saline-alkali soil improver, and a preparation method and applications thereof. The composite microbiological bacteriocin saline-alkali soil improver comprises a microbial agent, an organic liquid and a matrix. The microbial agent specifically comprises bacillus subtilis and bacillus megaterium, and nutrient substances of protease, lipase, alfa-amylase, amino acid and the like can be genereated by fermentation of the microbial bacteriocins; and the microbial agent can be used to adjust soil acidity-alkalinity, reduce saline-alkali toxicity, adjust rice root system microbial environment, accelerate transition of trace elements comprising nitrogen, phosphorus and potassium, and accelerate root growth, root increase, seedling development and seedling strengthening, and helps to lay a good foundation for increase of rice production.

Description

technical field [0001] The invention relates to the field of biological bacterial fertilizer technology and saline-alkali land treatment, in particular to a compound microbial bacterial element saline-alkali land improver and its preparation method and application. Background technique [0002] The ecological restoration and improvement of saline-alkali land is a project that benefits the country and the people. It can increase the area of ​​cultivated land, tap agricultural potential, ensure food production, and is more conducive to the development of the agricultural industry. It is conducive to expanding the grain planting area and ensuring the effective supply of grain. In Inner Mongolia, Jilin and other regions of China, good results have been achieved in saline-alkali land management. There are also many patents in saline-alkali land management. For example: Chinese patent CN101481281A (application date: 2009 February 3, application number: 200910066498.0, applicant: J...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/00C09K101/00
Inventor 陈昌和王楠刘嘉秦文斌刘国辉魏凌恺刘会欣
Owner TSINGHUA UNIV
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