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Preparation method of compound enzyme and probiotic preparation for feed through biotransformation of potato stem leaf and sunflower cake

A technology of potato stems and leaves and probiotic preparations, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult digestion of monogastric animals, achieve low-cost and high-efficiency biotransformation, extensive fermentation process, and strong enzyme production capacity Effect

Inactive Publication Date: 2014-12-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of potato stems and leaves, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Mix 20kg of the starter in step 1) with 600kg of potato stems and leaves, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 33.89IU / g; total cellulase activity reaches 4.77IU / g; beta-glucosidase activity reaches 33.89IU / g; reached 10.29IU / g; endoglucanase activity re...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of potato stems and leaves, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Mix 20kg of the starter in step 1) with 600kg of potato stems and leaves, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 33.63IU / g; total cellulase activity reaches 4.68IU / g; beta-glucosidase activity reaches 33.63IU / g; reached 10.15IU / g; endoglucanase activity rea...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of potato stems and leaves, 5kg of sunflower cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of potato stems and leaves, 300kg of sunflower cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 32.65IU / g; total cellulase activity reaches 5.05IU / g; beta-glucosidase activity reaches 5.05IU / g; reached 11.32IU / g; endoglucanase...

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Abstract

The invention discloses a preparation method of a compound enzyme and probiotic preparation for a feed through biotransformation of a potato stem leaf and a sunflower cake. The method comprises the following steps: fully mixing Pu-Er ripe tea having undergone pile fermentation, mushroom stick, agrocybe cylindracea stick, a first potato stem leaf, a first sunflower cake, first wheat bran, first apple pomace and first water and carrying out domestication and cultivation so as to obtain a leavening agent; and fully mixing the leavening agent, a second potato stem leaf, a second sunflower cake, second wheat bran, second apple pomace and second water and carrying out fermentation so as to obtain the compound enzyme and probiotic preparation for a feed. According to the invention, microecologically stable food-grade safe floras are used for fermentation of the potato stem leaf and the sunflower cake so as to prepare the compound enzyme and probiotic preparation with good performance indexes for the feed, and low-cost high-efficiency biotransformation of the potato stem leaf and the sunflower cake is realized. Meanwhile, the method provided by the invention has the advantages of easy growth of microbes, high enzyme activity, extensive fermentation process and easy realization of industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting potato stems and leaves and sunflower cake by adopting micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖李青青徐娟于骅诸文颖陈功杜姗姗杨璐纪晓燚吴渊
Owner ZHEJIANG UNIV
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