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Method for detecting aflatoxin B1 with high sensitivity in single-bead photonic crystal microsphere suspension array chemiluminescence way

A photonic crystal microsphere, aflatoxin technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of expensive detection instruments, large amount of reagents, and high detection costs, Achieve the effect of saving reagents and samples, low cost, and expanding the linear range of detection

Inactive Publication Date: 2013-10-09
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional detection methods have gradually shown shortcomings, such as high detection cost, large amount of reagents, expensive detection equipment, long detection time, etc.

Method used

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  • Method for detecting aflatoxin B1 with high sensitivity in single-bead photonic crystal microsphere suspension array chemiluminescence way
  • Method for detecting aflatoxin B1 with high sensitivity in single-bead photonic crystal microsphere suspension array chemiluminescence way
  • Method for detecting aflatoxin B1 with high sensitivity in single-bead photonic crystal microsphere suspension array chemiluminescence way

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Rice, corn and wheat are pulverized with a traditional Chinese medicine grinder, passed through a 200-mesh sieve, weighed 5g of each sample in a 100mL Erlenmeyer flask, added different concentrations and volumes of aflatoxin B1 standard substances to the sample, and mixed the sample thoroughly , put it in a ventilated place until the solvent evaporates completely. Add 15mL of extractant (methanol:water=4:6) to it, extract in a shaker at 150r / min for 2 hours, filter with whatman filter paper, and the filtrate is the sample to be tested. At the same time, extract the control sample without adding standard substance according to the above method.

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Abstract

The invention relates to a method for detecting aflatoxin B1 with high sensitivity in a single-bead photonic crystal microsphere suspension array chemiluminescence way. The method comprises the following steps of: adopting photonic crystal microspheres as a probe carrier of the aflatoxin B1 to perform competitive immunodetection to the aflatoxin B1 on the surfaces of the microspheres; performing competitive binding to a monoclonal toxin antibody through an antigen fixed to the surfaces of the microspheres, of the aflatoxin B1 in a sample; performing specific binding to the aflatoxin B1 antibody through a second antibody marked by horse radish peroxidase; and then detecting a chemiluminiscence light signal through a multifunctional microplate reader. According to the method, the aflatoxin detection limit to the aflatoxin B1 is 0.0001ng / mL, the linear detection range is 0.0001 to 1ng / ml, and only 2mu L sample is needed.

Description

technical field [0001] The invention designs a rapid, high-throughput and low-cost detection method for mycotoxins in agricultural products, feed and food. Specifically, the typical aflatoxin B1 in grains is used as the detection object, and the three-dimensional photonic crystal is used as the carrier, and a new liquid phase chip detection technology is constructed on the surface of single pearl photonic crystal microspheres through the competitive immunological method. Background technique [0002] Aflatoxin is a toxin discovered in the 1960s after an analysis of the sudden deaths of about 100,000 turkeys in the UK. Aflatoxin is a class of highly carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. Aflatoxins are widely distributed in nature, and are found in food crops such as rice, wheat, and corn, as well as milk, condiments, and edible oils. There are more than 20 known aflatoxins, among which aflatoxin B1 is the most toxic. ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/76
Inventor 李建林徐坤孙越李玮徐杰蒋云坤曹斌郑铁松
Owner NANJING NORMAL UNIVERSITY
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