Diatom carrier culture technique
A technology of diatom and carrier, applied in seaweed cultivation, cultivation, plant cultivation and other directions, can solve the problems of low biomass of diatom and large volume of aquaculture water, and achieve the effect of fast growth rate, promotion of cultivation efficiency and reduction of disease occurrence.
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Embodiment 1
[0018] Erlenmeyer flask culture of benthic diatoms
[0019] Cladophora was crushed and passed through a 20-mesh sieve. Seawater is filtered through a 0.22 μm microporous membrane, f / 2 medium is used, 0.5-2.0% sargassum powder is added to the water body, and activated benthic diatoms are inoculated, and the initial density of diatoms is 10 4 cell / ml, cultured at 23°C under continuous light and without ventilation, stirred 3 times a day, and cultured for 5-10 days. After the cultivation, the algae are separated by filtration, and each gram of wet weight contains 65 million diatom cells.
Embodiment 2
[0021] Reactor Cultivation of Benthic Diatoms
[0022] Sage algae was dried and crushed to 40 mesh. Seawater is filtered through a 0.45 μm microporous membrane, diatom-optimized medium is used, 0.1-5.0% Clado algae powder is added to the water body, and flat photobioreactors with optical paths of 30 mm, 50 mm and 100 mm are used for suspension culture. Inoculate activated benthic diatoms, the initial density of diatoms is 10 4 cell / ml, cultured at 23°C for 5-7 days under natural light and ventilation. After the cultivation, the algae are separated by static sedimentation, and each gram of wet weight contains more than 85 million diatom cells.
[0023] Add 0.1-5.0% Cladophora powder and an appropriate amount of diatom-optimized medium to the separated upper water body, carry out secondary culture in the original reactor under the same conditions, and obtain algae powder containing more than 80 million diatom cells per gram of wet weight.
[0024] The above-mentioned fresh an...
Embodiment 3
[0026] Pond cultivation of benthic diatoms
[0027] Sargassum dried and crushed through a 20-mesh sieve. The seawater is introduced into the culture pond (water depth 1.5m) after sand filtration, add 0.5-10% sargassum and appropriate amount of urea, inoculate the activated benthic diatoms, the initial density of diatoms is 10 3 cell / ml, cultured at natural temperature and natural light, ventilated twice a day in the morning and evening, 30 minutes each time, after 10-20 days of cultivation, the algae were drawn from the bottom of the pond, and after dewatering, each gram of wet weight contained 35 million diatom cells.
[0028] Put the above fresh bait directly into the water body to feed sea cucumbers. Under the same feeding mode, the survival rate of young ginseng is more than 50% higher than that of general feed, and the growth is good; adult ginseng has strong disease resistance, fast growth, and weight gain. The rate is high, which is 1.5-3.5 times faster than that of co...
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