Method for preparing beta-lactam derivative through enzymatic reaction

Abstract

The invention relates to a method for preparing beta-lactam derivative through enzymatic reaction. The method for preparing beat-lactam derivative through enzymatic reaction comprises the step of synthesizing a compound shown in a formula I by virtue of an enzymatic reaction, and the compound can be used for preparing antibacterial cefcapene pivoxil, wherein R is the conventional amino protection groups like a t-butyloxycarboryl group. A biological enzyme catalyst used in the enzymatic reaction is immobilized deacetyl esterase, and the method for synthesizing the compound shown in the formula I has the advantages of mild conditions, low cost and environmental friendliness, and is easy to operate.

Description

technical field

[0001] The invention relates to a method for preparing beta-lactam derivatives by enzymatic reaction, and the derivatives can be used to prepare antibacterial drug cefcapene pivoxil. Background technique

[0002] Cefcapene pivoxil is the fourth-generation oral cephalosporin antibiotic, developed by Shionogi Co., Ltd., Japan, and listed in 1997. Its chemical structure is as follows:

[0003]

[0004] It has a strong antibacterial effect on a variety of bacteria, stronger antibacterial activity than the existing oral cephalosporins, and a lower dosage.

[0005] There are many reports on the process route of cefcapene pivoxil in early patents and literature. The representative patent WO2008 / 155615 once disclosed the synthesis method of cefcapene pivoxil. -Acylation reaction of N atom, carbamylation reaction of 3-hydroxyl group, and esterification reaction of 4-carboxyl group to prepare cefcapene axetil. The preparation route is as follows:

[0006] [0...

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