Pretreatment kit and method for detecting niclosamide in aquatic products
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The technology of a niclosamide and kit is applied to the pretreatment kit and the field of testing niclosamide in aquatic products, which can solve the problems such as not seen, and achieve the advantages of less reagent amount, simple preparation and less reagent consumption Effect
Inactive Publication Date: 2013-11-20
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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At present, there are no reports of pretreatment kits for th...
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Embodiment 1
[0042] The present invention will be further described below in conjunction with the accompanying drawings.
[0043] A pretreatment kit for detecting niclosamide in aquatic products, the pretreatment kit of the present invention mainly consists of a box body 1, a first reagent bottle A2, a second reagent bottle B3, a third reagent bottle C4, and a fourth reagent bottle D5, fifth reagent bottle E6, sixth reagent bottle F7. The box body is divided into 6 areas a, b, c, d, e, and f, which are respectively suitable for the first reagent bottle A2, the second reagent bottle B3, the third reagent bottle C4, the fourth reagent bottle D5, and the fifth reagent bottle E6 . The hole area where the sixth reagent bottle F7 is placed, and the size order of the hole area is a>e=f>d>b>c.
[0044] Preferably, in the first reagent bottle A2, any one of ethyl acetate, acetone, acetonitrile, methanol, and dichloromethane has adjusted the acidic or alkaline liquid reagent of pH, and the liquid i...
Embodiment 2
[0051] A method for using a pretreatment kit for detecting niclosamide in aquatic products, the steps of which are:
[0052] A. Take 2.0-4.0g of edible tissues of aquatic products into a 15mL plastic centrifuge tube, add 2-10mL of liquid reagent in the first reagent bottle A2, and shake for 30s; the first reagent bottle A2 can be ethyl acetate, acetone, Any one of acetonitrile, methanol, and dichloromethane is an acidic (pH1-5) or alkaline (pH8-12) liquid reagent that adjusts the pH.
[0053] B. Then add 0.5-5g of the reagent in the second reagent bottle B3, shake for 30s, centrifuge at 5000r / min for 5min, and transfer the supernatant to a 15mL graduated centrifuge tube; the second reagent bottle B3 can be sodium chloride , sodium carbonate, potassium nitrate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ammonium dihydrogen phosphate, sodium sulfate or magnesium sulfate.
[0054] C. Add 2-6mL of the solution in the first reagent bottle A2 to the original ...
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Abstract
The invention discloses a pretreatment kit and method for detecting niclosamide in aquatic products. The kit comprises a kit body and reagent bottles which are respectively put in six empty areas of a, b, c, d, e and f in the kit body. The method comprises the following steps: A. putting a sample into a centrifugal tube, adding a first reagent, and oscillating; B. adding a second reagent, oscillating, centrifuging, and transferring supernate; C. adding the first reagent into the original centrifugal tube, oscillating, after centrifugation, taking the supernate out and mixing the supernate with the last supernate; D. metering the mixed liquid with a certain volume, after shaking uniformly, taking certain volume of liquid and putting into the centrifugal tube, and blow-drying with nitrogen; and E. dissolving residues with the solution of a third reagent, adding a fourth reagent, oscillating, centrifuging, taking the clear liquid and filtering, and carrying out HPLC-MS/MS(high performance liquid chromatography-mass spectrometry/mass spectrometry) measurement. The detecting method is simple, fast and accurate, high in sensitivity and low in cost, and is easy to popularize. The pretreatment kit is suitable for treatment of eatable samples of large amount of fresh and alive and ice aquatic products, and the pretreatment efficiency of the samples is greatly improved.
Description
technical field [0001] The invention belongs to the technical field of food safety analysis, and more specifically relates to a pretreatment kit for detecting Niclosamide (also applicable to Niclosamide ethanolamine salt) in edible tissues of aquatic products. At the same time, it also relates to a detection method for the residual amount of niclosamide (niclosamide ethanolamine salt also exists in the form of niclosamide after being processed by this kit) in edible tissues of aquatic products. This kit and detection method Applicable to the detection of niclosamide (also applicable to niclosamide ethanolamine salt) residues in fresh and chilled aquatic products (fish, shrimp) edible tissues (fish: muscle and skin, shrimp: muscle) Sample pretreatment and testing. Background technique [0002] Niclosamide, also known as snailicide, Yumosheng, shellfish kill, Mietailing, Qingtangjing, etc., is a highly efficient insecticide, and is also a synthetic high-efficiency molluscicid...
Claims
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IPC IPC(8): G01N30/06G01N30/88
Inventor 刘永涛艾晓辉索纹纹杨秋红胥宁杨红
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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