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Biosensor

一种生物传感器、绝缘性的技术，应用在生物传感器领域，能够解决技术难度高等问题，达到顺畅均匀扩散、克服边缘效应、提高准确性的效果

Active Publication Date: 2013-11-27
ACON BIOTECH (HANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the technical difficulty of improving the accuracy of biosensors to detect the concentration of glucose in human blood is very high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1 Adding Reagents to Electrodes Using Elliptical Reagent Diffusion Control Zones

[0095] Such as image 3 The shown biosensor includes an insulating substrate 1 , a working electrode 2 , a counter electrode 3 and a reference electrode 4 . The electrode system is covered with a reagent diffusion control layer 5, and the control layer 5 includes an elliptical reagent diffusion control zone 6, the opening area of ​​the reagent diffusion control zone 6 is the same as the reagent area to be added to the electrode and Just above the electrode active area. The biosensor described in this embodiment also includes a space manufacturing layer 7 and an upper cover 8 with vent holes.

[0096] In the biosensor manufacturing process, the reaction reagent is added to the electrode through the elliptical reagent diffusion control area by the method of pointing liquid. After the reaction reagents are added to the electrode system, use a digital stereo microscope (microscope ...

Embodiment 2

[0098] Example 2 Using a circular reagent diffusion control zone to add a reaction reagent on the electrode

[0099] Such as Figure 5 Shown is the reagent diffusion control layer 5 in which the reagent diffusion control region 6 is circular in shape. During the biosensor manufacturing process, the reaction reagent is added to the electrode through the circular reagent diffusion control area by the method of spotting liquid. After the reaction reagents are added to the electrode system, use a digital stereo microscope (microscope model MOTICAM 2306, manufacturer Macdio Industrial Group Co., Ltd.) to take pictures of the distribution of the reagents on the electrodes to observe the distribution of the reaction reagents on the electrodes picture. Such as Image 6 It is shown that the added reaction reagent diffuses uniformly on the working electrode 2, the counter electrode 3 and the reference electrode, and no reagent overflows the edge 6-1 defined by the reagent diffusion c...

Embodiment 3

[0101] Example 3 Addition of Reagents on Electrode System with Assisted Diffusion Electrode

[0102] Such as Figure 7 The electrode system of the shown biosensor comprises functional electrodes 2 , 3 , 4 and auxiliary electrodes 12 . The biosensor also includes a reagent diffusion control layer 5 with an oval 6 . In the biosensor manufacturing process, the reaction reagent is added to the electrode through the elliptical reagent diffusion control area by the method of pointing liquid. After the reaction reagents are added to the electrode system, use a digital stereo microscope (microscope model MOTICAM 2306, manufacturer Macdio Industrial Group Co., Ltd.) to take pictures of the distribution of the reagents on the electrodes to observe the distribution of the reaction reagents on the electrodes picture. Such as Figure 8 It is shown that the added reaction reagent diffuses evenly on the electrode, and no reagent overflows the edge 6-1 defined by the reagent diffusion con...

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Abstract

The invention provides a biosensor. The biosensor comprises an insulating base plate and an electrode system positioned on the insulating base plate, wherein reagents capable of reacting with a detection sample are contained on the electrode system; the electrode system comprises a functional electrode for detection and auxiliary diffusion electrodes for controlling diffusion of the reagents; the functional electrode is positioned among the auxiliary diffusion electrodes. The auxiliary diffusion electrodes can overcome the effect of surface tension of the insulating base plate, so that the reagents can be smoothly and uniformly diffused on the electrode system and reaches to preset positions.

Description

technical field [0001] The invention relates to a biosensor for detecting the content of physiological indexes. [0002] Background technique [0003] The field of healthcare testing has changed dramatically in recent years, from testing initially using clinical laboratory samples to rapid testing that can be done just in the doctor's office or next to the patient. Disposable biosensors with electrode systems are often used for rapid testing. Take the daily glucose detection of diabetic patients as an example. Now patients only need to use a disposable biosensor to detect at home, and then they can obtain the glucose level of the tested patient at that time, helping patients to know their physical condition in time and adjust their diet accordingly structure, or go to the hospital to let the doctor adjust the dosage according to the daily monitoring data. Therefore, the accuracy of such disposable biosensors is particularly important in the daily monitoring of diseases. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N33/5438G01N27/3272
Inventor 章子毅葛浣溪
Owner ACON BIOTECH (HANGZHOU) CO LTD
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