Turnover testing device and method for heavy metal availability by stimulating human digestion characteristics

Abstract

The invention provides a turnover testing device and method for heavy metal availability by stimulating human digestion characteristics. The method comprises the following steps of: (1) preprocessing a soil sample; (2) preparing simulated gastric fluid and intestinal fluid and heating; (3) simultaneously adding the preprocessed soil sample and the simulated gastric fluid into an extraction bottle and regulating the pH of the solution; (4) continuously overturning and oscillating; (5) at the end of oscillation, filtering with a filtering membrane, and analyzing the human availability of the heavy metal in a stomach stage by utilizing an ICP-MS (Inductively Coupled Plasma Mass Spectrometry); (6) after supplementing the fresh gastric fluid, adding the simulated gastric fluid, regulating the pH, and fixing the solution into an extraction chamber; (7) overturning and oscillating; (8) at the end of oscillation, filtering with the mixture to pass through the filtering membrane, and analyzing the human availability of the heavy metal in an intestine stomach stage by utilizing the ICP-MS. The testing method provided by the invention is simple in operation and large in testing capacity, requires few instruments, is capable of realizing automatic control and simultaneously testing 12 samples and can be widely used for testing the human availability of soil heavy metal in polluted sites.

Description

technical field [0001] The invention belongs to the field of environmental protection of human health testing in polluted sites, and in particular relates to an integrated device and method for flipping test of heavy metal human body availability in soil that simulates gastrointestinal digestion characteristics of human body. Background technique [0002] Due to inadequate environmental protection measures in the early smelting, metal product (such as electroplating, casting) processing and manufacturing, chromium salt and other production processes, the soil in the factory area of ​​these enterprises was polluted by heavy metals. For example, the site assessment of a chemical plant relocated in Beijing found that the As, Hg, and Cr contaminated soil in the plant area reached 31,000 m 3 . The evaluation of the soil of the Beijing Dyestuff Factory found that As and Cr contaminated soil reached 52,000 m 3 . An evaluation of an abandoned dye factory in Wuhan found that As co...

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Problems solved by technology

Although there are related invention patents in China, the test device is relatively complicated

For example, the multi-channel artificial simulating gastrointestinal system invented by Zhu Yongguan et al. The test system in the method of evaluating the human body effectiveness of soil pollutants includes argon gas, stirring, acid-base online titration, pH online monitoring and many other scattered subsystems. The operation is cumbersome and difficult. Control each sample in the same test environment

Lu Gaosheng et al. invented a simulated gastric juice extraction method for human-absorbable heavy metals in dust / soil, which only tested the availability of heavy metals in human gastric juice and did not test their availability in intestinal juice. The results are difficult to reflect objectively. actual human availability

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