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ft4 in vitro diagnostic kit and method of use thereof

A kit, FT4 technology, which is applied in biological testing, analysis by chemical reaction of materials, and material testing products, can solve problems such as poor hapten coating, achieve stability and extend the validity period, eliminate environmental and Hazards to operators, effects of getting rid of half-life limitations

Active Publication Date: 2016-02-24
上海裕隆医学检验所股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For those haptens with strong polarity and small molecular weight that cannot be coated on solid-phase carriers for detection, the present invention provides a convenient kit, which solves the problem of poor coating of haptens and facilitates rapid and sensitive detection. Detection of FT4

Method used

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  • ft4 in vitro diagnostic kit and method of use thereof
  • ft4 in vitro diagnostic kit and method of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Material:

[0054] Activation buffer: 0.1MMES, 0.5MNACl, pH6.0;

[0055] Carrier protein: BSA;

[0056] Target protein: small molecule hapten FT4;

[0057] NHS; Mercaptoethanol; Desalting column

[0058] Coupling the small molecule hapten FT4 with the carrier protein,

[0059] ① Equilibrate EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), NHS (N-hydroxysuccinimide) to room temperature.

[0060] ②Dissolve EDC with activation buffer, the final concentration is 50mg / mL, NHS is also dissolved with activation buffer, the final concentration is 50mg / mL, BSA (bovine serum albumin) is dissolved with activation buffer, the final concentration is 5mg / mL .

[0061] ③ After mixing 200uLEDC solution with 500uLBSA solution, add 200uL NHS solution, react at room temperature for 15 minutes, and then add 100uLEDC. React for another 15 minutes.

[0062] ④Add 1.4uL mercaptoethanol to terminate the EDC reaction and obtain the activated protein (EDC-BSA).

[0063] ...

Embodiment 2

[0087] Material:

[0088] Activation buffer: 0.1MMES, 0.5MNACl, pH6.0

[0089] Carrier protein: BSA;

[0090] Target protein: small molecule hapten FT4

[0091] NHS; Mercaptoethanol; Desalting column

[0092] Coupling the small molecule hapten FT4 with the carrier protein,

[0093] ① Equilibrate EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), NHS (N-hydroxysuccinimide) to room temperature.

[0094] ②Dissolve EDC with activation buffer, the final concentration is 45mg / mL, NHS is also dissolved with activation buffer, the final concentration is 55mg / mL, BSA (bovine serum albumin) is dissolved with activation buffer, the final concentration is 4.5mg / mL mL.

[0095] ③ After mixing 200uLEDC solution with 500uLBSA solution, add 200uL NHS solution, react at room temperature for 15 minutes, and then add 100uLEDC. React for another 15 minutes.

[0096] ④Add 1.4uL mercaptoethanol to terminate the EDC reaction and obtain the activated protein (EDC-BSA).

[0097...

Embodiment 3

[0119] Material:

[0120] Activation buffer: 0.1MMES, 0.5MNACl, pH6.0

[0121] Carrier protein: BSA;

[0122] Target protein: small molecule hapten FT4

[0123] NHS; Mercaptoethanol; Desalting column

[0124] The method of coupling the small molecule hapten FT4 and the carrier protein is as follows:

[0125] ① Equilibrate EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), NHS (N-hydroxysuccinimide) to room temperature.

[0126] ②Dissolve EDC with activation buffer, the final concentration is 55mg / mL, NHS is also dissolved with activation buffer, the final concentration is 45mg / mL, BSA (bovine serum albumin) is dissolved with activation buffer, the final concentration is 5.0mg / mL mL.

[0127]③ After mixing 200uLEDC solution with 500uLBSA solution, add 200uL NHS solution, react at room temperature for 15 minutes, and then add 100uLEDC. React for another 15 minutes.

[0128] ④Add 1.4uL mercaptoethanol to terminate the EDC reaction and obtain the activated p...

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PUM

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Abstract

The invention discloses an FT4 in vitro diagnostic kit. The invention provides a convenient kit for detecting those haptens with strong polarity and small molecular weight that cannot be coated on a solid-phase carrier, and solves the problem of hapten packaging. It is convenient for fast and sensitive detection of FT4 under poor conditions.

Description

technical field [0001] The invention relates to a FT4 detection kit, in particular to an FT4 in vitro diagnostic kit and a use method thereof. Background technique [0002] Free Thyroid Hormone (FT4) is one of the main indicators for evaluating thyroid function and studying the hypothalamus-pituitary-thyroid axis. FT4 is the biologically active part of thyroid hormone, which can directly reflect the state of thyroid function and is not affected by changes in the concentration and binding capacity of thyroxine-binding globulin (TBG) in blood. The measured value of FT4 was significantly higher than the normal range in hyperthyroidism; it was significantly lower than the normal range in hypothyroidism. Chronic active hepatitis and primary hepatic cirrhosis can also cause a decrease in FT4. After 36 weeks of gestation, FT4 normally decreases physiologically. [0003] FT4 has been using radioimmunoassay (RIA) before. RIA has both high specificity of immune response and high se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/78
Inventor 穆海东汪宁梅陈福美沈珏璟
Owner 上海裕隆医学检验所股份有限公司