Method for preparing ethanol with manioc wastes as raw materials through synergic saccharification fermentation

A technology of saccharification and fermentation and cassava residue, applied in biochemical equipment and methods, methods based on microorganisms, fermentation, etc., can solve the problems of high cost, separation and purification, high energy input, etc., to improve hydrolysis efficiency, improve saccharification effect, improve The effect of protein content

Inactive Publication Date: 2015-05-20
BEIJING FORESTRY UNIVERSITY +1
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Problems solved by technology

However, using these industrial wastes to produce ethanol, the maximum ethanol concentration in the fermentation broth is only close to 5%. The separation and purification of ethanol with this concentration requires high energy input, and the high cost of product separation and purification also limits the industrialization of industrial waste fermentation ethanol. an important reason

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  • Method for preparing ethanol with manioc wastes as raw materials through synergic saccharification fermentation

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Experimental program
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Effect test

Embodiment 1

[0028] 1) Add 1 kg of cassava dregs to 50°C water, keep it warm for 30 minutes, then add starch liquefying enzyme, and liquefy at 90°C for 2 hours under stirring conditions to obtain cassava dregs starch liquid; the weight volume percentage of cassava dregs and water is 15w / v %; The amount of starch liquefaction enzyme added is 8U / gram of cassava residue;

[0029] 2) Use 10% dilute sulfuric acid to adjust the pH value of the above tapioca dregs starch solution to 4.5, add starch glucoamylase, cellulase, and sapindus saponin, and saccharify at 48°C for 12 hours to obtain a saccharification solution; the amount of starch glucoamylase added is 60U / gram of cassava residue; the amount of cellulase added is 3.0FPU / gram of cassava residue; the weight volume percentage of sapinberry saponin and cassava residue starch liquid is 0.08w / v%;

[0030] 3) After adjusting the pH value of the cassava dregs saccharification solution to 6.0 with 10% sodium hydroxide, fill the saccharification so...

Embodiment 2

[0040] Ethanol was prepared by fermentation according to the method of Example 1. Among them, 1 kg of cassava residues is used as raw material, and the weight volume percentage of cassava residues and water is 23w / v%; the addition of starch liquefying enzyme is 10U / gram of cassava residues; the addition of starch glucoamylase is 70U / grams of cassava residues; fiber The amount of suzyme added is 4.0 FPU / gram of cassava dregs; the weight volume percentage of sapinberry saponin and tapioca dregs starch liquid is 0.12w / v%; w / v%; Alcoholic yeast is a high-temperature resistant type of alcoholic yeast, and the inoculum concentration is 7.5g / L.

Embodiment 3

[0049] Ethanol was prepared by fermentation according to the method of Example 1. Among them, 1 kg of cassava residues is used as raw material, and the weight volume percentage of cassava residues and water is 20w / v%; the addition amount of starch liquefying enzyme is 6U / per gram of cassava residues; the addition amount of starch glucoamylase is 50U / per gram of cassava residues; The amount of suzyme added is 1.0 FPU / gram of cassava dregs; the weight volume percentage of sapinberry saponin and tapioca dregs starch liquid is 0.01w / v%; w / v%; Alcoholic yeast is a high-temperature resistant type of alcoholic yeast, and the inoculum concentration is 5.5g / L.

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Abstract

The invention relates to a method for preparing ethanol with manioc wastes as raw materials through synergic saccharification fermentation. The manioc wastes are used as the raw materials to prepare the ethanol through liquification and cellulose-diastase saccharification fermentation. The ethanol preparation method of the invention can realize the production without using chemicals, and performs the resource utilization of the plurality of components in the manioc wastes to achieve high substrate concentration fermentation and shorten the fermentation time. Meanwhile, the relative amount of cellulose in fermentation residues after converting the manioc wastes through the ethanol is reduced, and the content of protein is improved, so that the method is more suitable for preparing pellet feeds (DDGS).

Description

technical field [0001] The invention relates to a new method for preparing ethanol by synergistic saccharification and fermentation with cassava residues as raw materials, belonging to the field of biomass chemistry and chemical industry. Background technique [0002] Human society in the world today is facing the crisis of energy depletion, and countries all over the world have invested a lot of money in the development of renewable energy. As a kind of renewable energy, fuel ethanol is currently the most widely used renewable motor fuel. Different regions use different raw materials for the production of fuel ethanol. For example, corn is used in the United States, and sugarcane is used in Brazil. China is a country with a large population. Using corn, wheat and other grains as raw materials to produce fuel ethanol will cause problems such as food security. Therefore, the government encourages the use of non-grain raw materials for the production of fuel ethanol. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14A23K1/14C12R1/865
CPCY02E50/10
Inventor 蒋建新唐勇雷福厚刘祖广朱莉伟
Owner BEIJING FORESTRY UNIVERSITY
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