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Silkworm pupa polypeptide skin-smoothing mask and preparation method thereof

A technology of silkworm chrysalis protein polypeptide and silkworm chrysalis, which is applied in the field of cosmetics and skin care products to achieve the effects of delaying skin aging, increasing added value, and low price

Active Publication Date: 2014-01-22
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007]1. The silk reeling process generally requires high temperature treatment, so silkworm chrysalis protein has a high degree of thermal denaturation and poor solubility. If it is directly applied to the preparation of cosmetics, it will not Easily absorbed by the skin; it must be converted into a soluble substance that is easily absorbed by the skin;
[0008]2. Silkworm chrysalis protein is a substance with high protein content. Since some people are allergic to high protein, if the protein contained in it is directly used in the cosmetics industry, it will also Cause allergies in some users

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0041] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 55°C, the pH value is 7.0, the enzymatic hydrolysis time is 3h, and the enzymatic hydrolysis is completed at 92°C Inactivate the enzyme for 13 minutes;

[0042] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;

[0043] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...

Embodiment 2

[0069] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0070] (1) Prepare a silkworm chrysalis protein solution with a concentration of 10% with deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 50°C, the pH value is 7.5, the enzymatic hydrolysis time is 4h, and the enzymatic hydrolysis is completed at 95°C Inactivate the enzyme for 10 minutes;

[0071] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.5, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 50°C, and the enzyme is extinguished at 95°C after the enzymatic hydrolysis is completed. Enzyme 10min;

[0072] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...

Embodiment 3

[0080] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide

[0081] (1) Prepare silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, enzyme amount 60000IU / g silkworm chrysalis protein, 3%, enzymolysis temperature 45℃, pH value 6.5, enzymolysis time 2h, enzymolysis is completed Then inactivate the enzyme at 90°C for 15 minutes;

[0082] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 10.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 45°C, and the enzymatic hydrolysis is extinguished at 90°C Enzyme 15min;

[0083] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfi...

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Abstract

The invention relates to the technical fields of cosmetics and skin care products, particularly a silkworm pupa polypeptide skin-smoothing mask which comprises 5-10 parts of silkworm pupa protein polypeptide, 1-2 parts of rosemary essential oil, 1-3 parts of pomegranate seed extract, 1-6 parts of sea algae extract, 3-6 parts of glycerol, 1-4 parts of olive oil, 2-5 parts of caprylic / capric triglyceride and 1-3 parts of pearl powder. The silkworm pupa protein polypeptide is prepared by carrying out enzymolysis on the silkworm pupa protein by papain and Esperase proteinase. The combination of the active silkworm pupa polypeptide, rosemary extract, pomegranate seed extract and other active substances can help the skin to resist injury from free radicals and collagenase, thereby effectively preventing and resisting wrinkles from generation, enhancing the skin elasticity and postponing aging. The mask prepared from the silkworm pupa protein and plant extracts is natural and mild, and does not stimulate the skin; and under the synergetic effect of multiple active substances on the skin, the mask provided by the invention has better effects than common masks.

Description

[0001] technical field [0002] The invention relates to the technical field of cosmetics and skin care products, in particular to a silkworm chrysalis polypeptide skin rejuvenating mask, and also relates to a preparation method of the silkworm chrysalis polypeptide skin rejuvenating mask. Background technique [0003] silkworm chrysalis( silkworm pupa ), the chrysalis of Bombyx mori, the silkworm moth, is the main by-product of the silk industry. my country is a big country in the silk industry. There are hundreds of thousands of tons of leftovers from silk reeling factories every year. Silkworm chrysalis resources are extremely rich. However, due to the lack of deep processing technology, most silkworm chrysalis are used as feed or fertilizer, or even discarded directly. The resource utilization rate and The added value is low, resulting in great waste. [0004] Silkworm chrysalis contains a variety of active substances, among which the protein content of silkworm chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/97A61K8/92A61K8/64A61Q19/00A61Q19/08
Inventor 刘玮杜芳岭陈相艳刘丽娜徐同成陶海腾邱斌
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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