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Determination method of content of active ingredient of Pingxiao capsule

A determination method and technology of Pingxiao Capsule, applied in the field of medicine, can solve the problems of hidden dangers in production of patients, inability to truly reflect product quality, and uncontrollable product stability.

Active Publication Date: 2014-01-29
XIAN C P PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prescription of Pingxiao Capsules is Curcuma 54g Agrimony 54g Wulingzhi 45g Alumen 54g Saltpeter 54g Dry paint (manufactured) 18g Aurantium Fructus (stir-fried bran) 90g Nuxychia powder 36g, Aurantii Fructus has the most content and is the most important component , the content determination method in the above Pharmacopoeia only measures the content of strychnine in Nuxychia, which cannot truly reflect the quality of the product, so that the stability of the product cannot be controlled, which brings hidden dangers to production and patients

Method used

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  • Determination method of content of active ingredient of Pingxiao capsule
  • Determination method of content of active ingredient of Pingxiao capsule
  • Determination method of content of active ingredient of Pingxiao capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Get 54 grams of medicinal materials turmeric, 54 grams of Agrimony, 45 grams of Wulingzhi, 54 grams of alum, 54 grams of saltpeter, 18 grams of dried lacquer, 90 grams of bran-fried Fructus Aurantii, 36 grams of Nuxynum powder; smell, dry lacquer, wulingzhi, alum, and saltpeter are pulverized into fine powder; turmeric and bran fried Fructus Aurantii are pulverized into the coarsest powder, use 70% ethanol as solvent, carry out percolation, collect 600ml of percolation liquid, reclaim ethanol, Standby; decoct the dregs and Agrimony with water twice, filter, combine the filtrate and combine with the above percolate, concentrate under reduced pressure to form a thick paste, dry, add nuxe seed powder, the above fine powder and an appropriate amount of starch, and mix well , granulated, dried, packed into capsules, and made into 1000 grains, that is to say.

[0088] Assay method

[0089] (1) Preparation of reference substance: Accurately weigh an appropriate amount of nari...

Embodiment 2

[0095] Reference substance solution was prepared according to Example 1

[0096] Need testing solution: take 20 capsules of Pingxiao Capsules of Example 1, put them in a mortar and grind them through a No. 4 sieve, take about 0.5g, accurately weigh them, put them in a stoppered Erlenmeyer flask, and precisely add 25ml of ethanol for reflux extraction treatment After 30 minutes, let it cool, filter, concentrate and dry under reduced pressure, then dissolve it with 25ml of methanol and dilute it to a 25ml volumetric flask, accurately measure 5ml of the subsequent filtrate, put it in a 25ml volumetric flask, dilute to the mark with methanol, and shake well. Instantly.

[0097] Chromatographic conditions: WatersC 18 Column 4.6×250mm, 5μm, mobile phase: acetonitrile-water=15:85, adjust pH value to 2.0 with 20% phosphoric acid; detection wavelength is 283nm; column temperature: room temperature; flow rate: 1.2ml·min -1 ;Injection method: automatic injection.

[0098] Determinatio...

Embodiment 3

[0100] Reference substance solution was prepared according to Example 1

[0101] Need testing solution: get 20 Pingxiao Capsules of Example 1, put them in a mortar and grind them through a No. 4 sieve, get about 0.5g, accurately weigh them, put them in a stoppered Erlenmeyer flask, add 25ml of methanol precisely, weigh them Weight, sonicate for 40 minutes, let cool, weigh again, make up the lost weight with methanol, shake well, filter, discard the initial filtrate, accurately measure 5ml of the follow-up filtrate, put it in a 25ml measuring bottle, and dilute with methanol To the scale, shake well, that is.

[0102] Chromatographic conditions: WatersC 18 Column 4.6×250mm, 5μm, mobile phase: acetonitrile-water=30:70, adjust pH value to 5.0 with 5% phosphoric acid; detection wavelength is 283nm; column temperature: room temperature; flow rate: 0.5ml·min -1 ;Injection method: automatic injection.

[0103] Determination method Accurately draw 10 μl each of the reference substa...

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Abstract

The invention relates to a determination method of the content of an active ingredient of a Pingxiao capsule. The determination method includes preparation of a reference solution, preparation of a test solution and formulation of high performance liquid chromatography conditions, wherein naringin content is not less than 2.13mg / particle and neohesperidin content is not less than 1.22mg / particle. The determination method is simple, safe and reliable, and is capable of faithfully representing product content and quality; the determination method is suitable for industrial daily production and inspection.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for determining the content of active ingredients in Pingxiao capsules. Background technique [0002] Existing Chinese patent medicine Pingxiao Capsule is a kind of commonly used antitumor Chinese patent medicine. [Content Determination] of Pingxiao Capsules recorded in Pharmacopoeia and Ministry Standards: Take 30 capsules of this product, pour out the contents, accurately weigh, grind finely, take about 4g of powder, accurately weigh, put in a 100ml conical flask with a stopper Add 20ml of chloroform and 1ml of concentrated ammonia solution precisely, seal it tightly, weigh it, soak it in cold for 48 hours, or extract it by ultrasonic for 40 minutes, weigh it, make up the lost weight with chloroform, shake it fully, filter it, and measure it precisely Filtrate 10ml, put in separatory funnel, extract 4 times with sulfuric acid solution (0.5mol / L), each 10ml, extract is all filt...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 宋强杜宏伟何莉李鹏安毅葛亚宁孙艳霞
Owner XIAN C P PHARMA
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