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Method for measuring content of active ingredients of Pingxiao tablets

A determination method and technology of active ingredients, applied in the field of medicine, can solve problems such as uncontrollable product stability, hidden dangers in production and patients, and inability to truly reflect product quality

Active Publication Date: 2014-01-29
XIAN C P PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prescription of Pingxiao Tablets is turmeric 54g Agrimony 54g Wulingzhi 45g Alumen 54g Saltpeter 54g Dry paint (made) 18g Citrus aurantium (stir-fried with bran) 90g Nuxychia powder 36g, Citrus aurantium has the most content and is the most important component , the content determination method in the above Pharmacopoeia only measures the content of strychnine in Nuxychia, which cannot truly reflect the quality of the product, so that the stability of the product cannot be controlled, which brings hidden dangers to production and patients

Method used

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  • Method for measuring content of active ingredients of Pingxiao tablets
  • Method for measuring content of active ingredients of Pingxiao tablets
  • Method for measuring content of active ingredients of Pingxiao tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Take 54 grams of curcuma curcuma, 54 grams of Agrimony, 45 grams of Wulingzhi, 54 grams of alum, 54 grams of saltpeter, 18 grams of dried lacquer, 90 grams of bran-fried Fructus Aurantii, and 36 grams of Nuxynum powder; Dried lacquer, wulingzhi, alum, and saltpeter were crushed into fine powder; turmeric and bran fried Fructus Aurantii were crushed into the coarsest powder, and 70% ethanol was used as solvent to percolate, and 600ml of percolation liquid was collected; Decoct twice with water, filter, and combine the decoction; after recovering ethanol from the percolation liquid, combine with the above filtrate, concentrate under reduced pressure to form a thick paste, dry, grind into fine powder, add nuxe seed powder, the above fine powder and auxiliary materials Appropriate amount, mixed evenly, granulated, dried, compressed into 1000 tablets, coated with sugar or film, and ready to be obtained.

[0086] Assay method

[0087] (1) Preparation of reference substance: ...

Embodiment 2

[0094] The reference substance solution was prepared according to Example 1.

[0095] Need testing solution: take 20 tablets of Pingxiao Tablets of Example 1, put them in a mortar and grind them finely through a No. 4 sieve, take about 0.5g, accurately weigh them, put them in a stoppered Erlenmeyer flask, and add 25ml of ethanol to them for reflux extraction. After 30 minutes, let it cool, filter, concentrate and dry under reduced pressure, then dissolve it with 25ml of methanol and dilute it to a 25ml volumetric flask, accurately measure 5ml of the subsequent filtrate, put it in a 25ml volumetric flask, dilute to the mark with methanol, and shake well. Instantly.

[0096] Chromatographic conditions: WatersC 18 Column 4.6×250mm, 5μm, mobile phase: acetonitrile-water=15:85, adjust pH value to 2.0 with 20% phosphoric acid; detection wavelength is 283nm; column temperature: room temperature; flow rate: 1.2ml·min -1 ;Injection method: automatic injection.

[0097] Assay

[009...

Embodiment 3

[0100] The reference substance solution was prepared according to Example 1.

[0101] Need testing solution: get 20 tablets of Pingxiao Tablets of Example 1, put them in a mortar and grind them through a No. 4 sieve, get about 0.5g, accurately weigh them, put them in a stoppered Erlenmeyer flask, add 25ml of methanol precisely, weigh them Weight, sonicate for 40 minutes, let cool, weigh again, make up the lost weight with methanol, shake well, filter, discard the initial filtrate, accurately measure 5ml of the follow-up filtrate, put it in a 25ml measuring bottle, and dilute with methanol To the scale, shake well, that is.

[0102] Chromatographic conditions: WatersC 18 Column 4.6×250mm, 5μm, mobile phase: acetonitrile-water=30:70, adjust pH value to 5.0 with 5% phosphoric acid; detection wavelength is 283nm; column temperature: room temperature; flow rate: 0.5ml·min -1;Injection method: automatic injection.

[0103] Assay

[0104] Accurately draw 10 μl each of the referen...

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Abstract

The invention relates to a method for measuring content of active ingredients of Pingxiao tablets. The method comprises the steps of preparing a reference solution and a test solution and setting conditions of high performance liquid chromatography, wherein the naringin content is not less than 2.26mg per tablet, and the neohesperidin content is not less than 1.33mg per tablet. The method is simple, safe and reliable, can really reflect the content and quality of the product and is suitable for industrialized daily production testing.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a content determination method of active ingredients. Background technique [0002] The existing Chinese patent medicine Pingxiao Tablet is a commonly used anti-tumor Chinese patent medicine. [Content Determination] of Pingxiao Tablets recorded in Pharmacopoeia and Ministry Standards Take 30 tablets of this product, remove the coating, accurately weigh, grind finely, take about 4g of powder, accurately weigh, put in a 100ml conical flask with a stopper , accurately add 20ml of chloroform and 1ml of concentrated ammonia solution, seal tightly, weigh, soak in cold for 48 hours, or extract by ultrasonic for 40 minutes, weigh, make up the lost weight with chloroform, shake fully, filter, and accurately measure the filtrate 10ml, put in a separatory funnel, extract 4 times with sulfuric acid solution (0.5mol / L), 10ml each time, filter the extract into a 50ml measuring bottle with filter paper...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 宋强杜宏伟何莉李鹏安毅葛亚宁孙艳霞
Owner XIAN C P PHARMA
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