Method for preparing stingray extract and application thereof in preparation of alcoholic liver protecting medicament

Abstract

The invention provides a method for preparing stingray extract. The method is characterized by comprising the following steps: steaming fresh stingray, and removing the flesh; flushing cartilage with clean water, and mincing; adding NaOH solution according to a material-solution mass ratio of 1:(1-1.5), stirring and extracting for 4 hours; regulating the pH to 6-7 with HCl; filtering, and adding NaOH into the filtrate to regulate the pH to 8.5-9.0; adding compound protease into the liquid according to a mass ratio of liquid to compound protease being 1,000:1, and hydrolyzing for 4 hours; heating the hydrolysate at 80 DEG C for 30 minutes, adding HCl to regulate the pH to be 2, and standing; carrying out suction filtration on upper solution with diatomite, and adding NaOH to regulate the pH to 10.5; decoloring with hydrogen peroxide, and filtering; adding HCl into the filtrate to regulate the pH to 7; concentrating the filtrate to a small volume under reduced pressure; and precipitating and drying to obtain the stingray extract. The stingray extract can increase the capability of a body in cleaning peroxidation products by strengthening the activity of antioxidant enzyme, and can be used as an alcoholic liver injury protecting agent.

Description

technical field [0001] The invention relates to a preparation method of a stingray extract and its application in the preparation of a drug for protecting alcoholic liver damage. Background technique [0002] At present, alcoholism and addiction have become a global problem. Long-term heavy drinking can cause damage to multiple organs throughout the body, especially to the liver. Chronic alcoholism can lead to symptoms such as alcoholic fatty liver, alcoholic hepatitis, alcoholic cirrhosis, fibrosis, and in severe cases, canceration. At present, for the treatment of alcoholic liver poisoning, many methods include amino acid and glucose infusion, multivitamins, maintenance of water, electrolyte and acid-base balance, etc. In recent years, many researchers have tried to find a method that can effectively prevent and protect the liver from Medicines that lessen the damaging effects of alcohol on the liver. [0003] Stingrays, also known as devil rays, are the same kind of sha...

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation method of stingray extract and its application in the preparation of alcoholic liver injury protection drugs.