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A kind of extraction method of chlorophyll in loofah leaf

An extraction method and chlorophyll technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve problems such as waste of active ingredients in loofah branches and leaves, improve extraction efficiency, prevent degradation of decomposition activity, and reduce production costs. Effect

Active Publication Date: 2016-03-16
ANHUI FINDSHE BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wherein loofah is not utilized as a kind of vegetable general branch and leaf, but contains a large amount of active ingredient substances in the loofah branch and leaf, contains abundant chlorophyll at the same time, has good health care effect, has therefore caused a large amount of waste of active ingredient in the loofah branch and leaf

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Grind the loofah leaves, add 80% aqueous ethanol at a ratio of 1:20, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.03% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 70° C. to finally obtain chlorophyll extract.

Embodiment 2

[0015] Grind the loofah leaves, add 60% aqueous ethanol at a ratio of 1:40, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.05% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 90° C. to finally obtain the chlorophyll extract.

Embodiment 3

[0017] Grind the loofah leaves, add 70% aqueous ethanol at a ratio of 1:30, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.04% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 80° C. to finally obtain the chlorophyll extract.

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PUM

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Abstract

The invention discloses a method for extracting chlorophyll from loofah leaves. The method uses ethanol containing water as an extractant and adopts ultrasonic-assisted extraction technology to extract natural chlorophyll from loofah leaves. The specific process flow is as follows: the loofah leaves are ground According to the solid-liquid ratio of 1:20-40, add 60%-80% aqueous ethanol and use ultrasonic-assisted leaching, filter the extract and carry out vacuum distillation and concentration to obtain chlorophyll extract. The invention changes the original extraction process of harmful solvents such as acetone, reduces industrial pollution, replaces the original toxic reagent with ethanol, not only effectively improves the extraction efficiency, but also greatly reduces the harm of harmful substances to the human body.

Description

technical field [0001] The invention relates to the field of chemical extraction, in particular to a method for extracting chlorophyll in loofah leaves. Background technique [0002] Chlorophyll is the main pigment for photosynthesis in plants. It is a lipid-containing pigment family located in the thylakoid membrane. Chlorophyll is a magnesium porphyrin compound, including chlorophyll a, b, c, d, f, prochlorophyll and bacteriochlorophyll. Chlorophyll is not very stable, light, acid, alkali, oxygen, oxidant, etc. will cause it to decompose. Under acidic conditions, chlorophyll molecules can easily lose the magnesium in the porphyrin ring and become pheophyllin. Chlorophyll widely exists in most green plants, and has multiple functions such as hematopoiesis, beauty and anti-virus, but most of the extraction of chlorophyll still adopts the method of acetone and ether, and these two organic reagents are very toxic in themselves. The human body will also cause certain harm, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/22C09B61/00
CPCC07D487/22C09B61/00
Inventor 夏省云
Owner ANHUI FINDSHE BIOLOGICAL PHARMA CO LTD
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