The invention provides a preparation method of articaine hydrochloride. The preparation method comprises the following steps of: reacting methyl mercaptoacetate with 2-metyl acrylonitrile used as starting raw materials in a mixed solution of sodium methoxide and methanol, after the reaction is finished, adjusting the pH value to 7 with concentrated hydrochloric acid, dropwise adding hydrogen peroxide, then dropwise adding concentrated hydrochloric acid to the reaction system, and concentrating under reduced pressure to obtain an intermediate compound B; dissolving the compound B in dichloromethane containing pyridine, dropwise adding alpha-chloropropionyl chloride, washing the reaction product, drying and concentrating to obtain an intermediate compound C; and dissolving the compound C with dimethyl sulfoxide, dropwise adding to a dimethyl sulfoxide solution containing n-propylamine, reacting at room temperature, mixing the product with ice water, extracting with ethyl acetate, drying, concentrating to obtain an oil product, dissolving with acetone, adjusting the pH value to 5-6 with concentrated hydrochloric acid, crystallizing, filtering and drying to obtain a crude product of the articaine hydrochloride. The method has the advantages that the operation is easy, the separation and purification are convenient, the product yield is high, the product purity is more than 99%, the industrial pollution is low, and the method is suitable for mass production.