Method for extracting chlorophyll from luffa leaf

An extraction method, chlorophyll technology, applied to chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of waste of active ingredients in the branches and leaves of loofah, improve extraction efficiency, prevent degradation of decomposition activity, and reduce industrial pollution Effect

Active Publication Date: 2014-03-12
ANHUI FINDSHE BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wherein loofah is not utilized as a kind of vegetable general branch and leaf, but contains a large amount of active ingredient substances in the loofah branch and leaf, contains abundant chlorophyll at the same time, has good health care effect, has therefore caused a large amount of waste of active ingredient in the loofah branch and leaf

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Grind the loofah leaves, add 80% aqueous ethanol at a ratio of 1:20, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.03% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 70° C. to finally obtain chlorophyll extract.

Embodiment 2

[0015] Grind the loofah leaves, add 60% aqueous ethanol at a ratio of 1:40, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.05% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 90° C. to finally obtain the chlorophyll extract.

Embodiment 3

[0017] Grind the loofah leaves, add 70% aqueous ethanol at a ratio of 1:30, and use ultrasonic-assisted extraction. The ultrasonic frequency is 20kHz, the power is 40w, the ultrasonic time is 15min, and 0.04% of For cellulase, the extraction solution is filtered and then concentrated by vacuum distillation. The temperature of the vacuum distillation is 80° C. to finally obtain the chlorophyll extract.

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Abstract

The invention discloses a method for extracting chlorophyll from a luffa leaf. The method comprises the following steps: using hydrous ethanol as an extractant, and extracting natural chlorophyll from the luffa leaf through adopting an ultrasonic-assisted extraction technology. The specific technological processes are that the luffa leaf is ground, 60-80 % hydrous ethanol is added according to the feed liquid ratio of 1:(20-40), and through the adoption of the ultrasonic-assisted extraction technology, the extract liquid is subjected to reduced pressure distillation after being filtered to obtain the chlorophyll extract. Through the adoption of the method, the original extracting technology of harmful solvents such as acetone is changed, the industrial pollution is reduced, and ethanol replaces original toxic solvents, so that not only is the extracting efficiency improved effectively, but also the injury to a human body of harmful substances is reduced greatly.

Description

technical field [0001] The invention relates to the field of chemical extraction, in particular to a method for extracting chlorophyll in loofah leaves. Background technique [0002] Chlorophyll is the main pigment for photosynthesis in plants. It is a lipid-containing pigment family located in the thylakoid membrane. Chlorophyll is a magnesium porphyrin compound, including chlorophyll a, b, c, d, f, prochlorophyll and bacteriochlorophyll. Chlorophyll is not very stable, light, acid, alkali, oxygen, oxidant, etc. will cause it to decompose. Under acidic conditions, chlorophyll molecules can easily lose the magnesium in the porphyrin ring and become pheophyllin. Chlorophyll widely exists in most green plants, and has multiple functions such as hematopoiesis, beauty and anti-virus, but most of the extraction of chlorophyll still adopts the method of acetone and ether, and these two organic reagents are very toxic in themselves. The human body will also cause certain harm, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22C09B61/00
CPCC07D487/22C09B61/00
Inventor 夏省云
Owner ANHUI FINDSHE BIOLOGICAL PHARMA CO LTD
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