Composition for increasing artemisinin content in Artemisia annua and application method thereof

Abstract

The invention discloses a composition for increasing the content of artemisinin in Artemisia annua and its application method; the composition includes abscisic acid and methyljasmonic acid; the concentration of abscisic acid in the composition is 1-50 μM, form The concentration of jasmonic acid is 1-50 μM. When applying, the Artemisia annua plants are sprayed with the composition 8-12 days before the plants grow to bloom. That's it. The active ingredient abscisic acid and methyl jasmonic acid in the composition of the present invention have a significant synergistic effect, which can increase the expression of more important genes in the artemisinin synthesis pathway, thereby leading to a greater increase in the content of artemisinin ; Simultaneously, because abscisic acid and methyl jasmonic acid are widely sourced, and the price is low, therefore have broad market prospect.

Description

technical field [0001] The invention relates to a composition in the field of biotechnology, in particular to a composition for increasing the content of artemisinin in Artemisia annua and its application method. Background technique [0002] Malaria is one of the major diseases of human health, causing 650,000 deaths every year. Artemisinin (artemisinin, a sesquiterpene lactone) extracted from the plant of Artemisia annua L. native to my country is currently the most effective antimalarial drug, and the combination therapy with artemisinin as the main raw material drug (Artemisinin-CombinationTherapy) is recommended by the World Health Organization as the treatment of choice for malaria. The annual global demand for artemisinin is about 140-200 tons per year, and it is increasing at a rate of 10-20% per year. However, the content of artemisinin in Artemisia annua is very low (artemisinin has the highest content in the leaves, but only accounts for 0.01% to 1% of the dry we...

AI Technical Summary

Problems solved by technology

However, the content of artemisinin in Artemisia annua is very low (artemisinin has the highest content in the leaves, but only accounts for 0.01% to 1% of the dry weight of the leaves), which leads to its high price. In recent years, its price has been 2300 ~3200 yuan / kg, but it is on the rise with the growth of demand

Although the production of artemisinin by genetically modified yeast is now possible, due to the limitation of production capacity, it cannot meet the global demand for artemisinin. How to find a simple and efficient method to increase the content of artemisinin in Artemisia annua is of great significance to reduce its production cost

[0003] Artemisinin is a plant secondary metabolite. In recent years, scholars at home and abroad have tried to use some plant hormones or stress factors that can stimulate the production of plant secondary metabolites to treat Artemisia annua, which can indeed increase the content of artemisinin in Artemisia annua. For example, using salt, salicylic acid, etc. to treat Artemisia annua at different stages of growth can indeed increase the content of artemisinin to a certain extent, but the effect of the increase is not significant, and the mechanism of increasing the content of artemisinin after treatment is also different. not very clear

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