Method for cultivating sweet wormwood herb with high artemisinin content by co-transforming genes Anti-Dxr and Anti-Fpps

A technology of co-transformation and artemisinin, applied in the field of genetic engineering to cultivate plants, can solve the problems of low toxicity and limited drug effect

Inactive Publication Date: 2015-04-22
CHONGQING MEDICAL & PHARMA COLLEGE
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Problems solved by technology

The drug resistance of Plasmodium limits the effect of the antimalarial drug chloroquine, while artemisinin has the characteristics of low toxicity and quick action against malaria

Method used

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  • Method for cultivating sweet wormwood herb with high artemisinin content by co-transforming genes Anti-Dxr and Anti-Fpps
  • Method for cultivating sweet wormwood herb with high artemisinin content by co-transforming genes Anti-Dxr and Anti-Fpps
  • Method for cultivating sweet wormwood herb with high artemisinin content by co-transforming genes Anti-Dxr and Anti-Fpps

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but it should not be understood that the scope of the subject matter of the present invention is limited to the following embodiments. Without departing from the above-mentioned technical ideas of the present invention, various replacements and changes made according to common technical knowledge and conventional means in this field shall be included in the protection scope of the present invention.

[0020] Materials: The conventional wild Artemisia annua with high artemisinin content was used, which was sourced from Xiushan County, Chongqing in 2008. It was routinely bred for 5 generations in Chongqing Medical College with the goal of high artemisinin.

[0021] Strains and plasmids: E.coli JM109, pMD 18-T vector, plasmid vector pBI121, plasmid vector pCAMBIA2301, Agrobacterium tumefaciens C58C1.

[0022] Enzymes and chemical reagents: DNA Taq polymerase...

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Abstract

The invention discloses a method for cultivating sweet wormwood herb with high artemisinin content by co-transforming genes Anti-Dxr and Anti-Fpps. The method comprises the following steps: guiding two genes namely Dxr and Fpps into sweet wormwood herb by virtue of a plant expression vector, and screening to obtain sweet wormwood herb with high artemisinin content in flower buds. And the method comprises the following specific steps: cloning a Dxr gene and an Fpps gene from sweet wormwood herb, constructing the plant expression vector containing the two genes namely the Dxr gene and the Fpps gene, performing mediation by using agrobacterium tumefaciens, transferring the Dxr gene and the Fpps gene into sweet wormwood herb, and cultivating and screening regenerated plants to obtain a transgenic sweet wormwood herb plant with increased artemisinin content in the flower buds. When the CK content of non-transformed comparison sweet wormwood herb is 6.20mg/g of dry weight, the maximum artemisinin content in the flower buds of an obtained gene engineering sweet wormwood herb plant disclosed by the invention is 17.40mg/g of dry weight, and the artemisinin content in the flower buds is 2.81 times of the content of non-transformed comparison sweet wormwood herb. The method disclosed by the invention has great significance in saving costs for pharmaceutical factories which take sweet wormwood herb as a raw material.

Description

technical field [0001] The invention relates to the field of cultivating plants by genetic engineering, in particular to a method for cultivating Artemisia annua with high artemisinin content by using transgenic technology. Background technique [0002] Artemisiae annie L., Asteraceae, Artemisia. The scientific name is Artemisia annua, also known as Artemisia stinky, Artemisia annua, and Artemisia annua. It grows on hillsides, forest margins and wastelands, and is an annual herb. Ordinary people use it to treat summer heat, headache, fever and cold. The extraction of artemisinin is to use the aerial parts of Artemisia annua and unopened flower buds to extract and produce effective physiologically active ingredients such as artemisinin and other products, which are low-toxic , efficient and quick-acting new antimalarial drug (Chinese Pharmacopoeia, 2005; Zhong Guoyue, et al., 2007). Artemisia annua is rich in wild resources, but the content of artemisinin is not high, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/61C12N15/54A01H5/00
Inventor 陈俊意朱照静杨治国杨延音谭丽田数高管琴张宝勇彭坤曾祥琼
Owner CHONGQING MEDICAL & PHARMA COLLEGE
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