A method for improving artemisinin content in Artemisia annua by transfecting iaam gene

Abstract

The invention discloses a method of improving content of artemisinin in artemisia annua by a trans-iaaM gene. The method comprises the following steps: constructing a carrier of the iaaM gene which is specifically expressed in a gland hair cell of artemisia annua, and converting the artemisia annua by means of an agrobacterium tumefaciens mediation method to obtain a transgenetic artemisia annua plant through PCR (Polymerase Chain Reaction) detection; carrying out PCR gene amplification detection on the transgenetic artemisia annua plant and observing and analyzing condition of glandular hair cell of transgenetic artemisia annua and content of artemisinin; screening the transgenetic artemisia annua plant with the content of artemisinin being remarkably improved; and establishing a transgenetic artemisia annua strain with high content of artemisinin through propagation by tissue culture. The content of artemisinin in the transgenetic artemisia annua obtained by the method disclosed by the invention is remarkably improved, reaching 1.5 times of that of uninverted artemisia annua at the highest. The method disclosed by the invention is significant for providing high-yielding and stable new medicine sources to scaled production of artemisinin.

Description

Technical field

[0001] The invention relates to a method for increasing the content of artemisinin belonging to the field of biotechnology, in particular to a method for increasing the content of artemisinin in Artemisia annua by transducing iaaM genes. Background technique

[0002] Artemisinin is a peroxy group-containing sesquiterpene lactone drug extracted from the plant Artemisia annua. It is the most ideal drug for malaria treatment today and can effectively treat Plasmodium infections that are resistant to the traditional drug chloroquine. Artemisinin is mainly obtained by directly extracting from Artemisia annua, or by extracting artemisinic acid, which has a higher content in Artemisia annua, and then semi-synthesized. Except for Artemisia annua and Artemisia annua, no other natural plant resources containing artemisinin have been discovered. Therefore, Artemisia annua is still the only drug source for the production of artemisinin drugs.

[0003] Although Artemisia annua...