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Composition for increasing artemisinin content in artemisia apiacea and preparation method thereof

A composition and artemisinin-containing technology are applied in the field of compositions and preparations for increasing the content of artemisinin in Artemisia annua, which can solve the problems of low feasibility and difficulty in realizing commercial production, and achieve low price, Significant synergy, wide-ranging effects

Inactive Publication Date: 2017-05-24
CHONGQING DINGSHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of artemisinin in the callus of Artemisia annua is less than 0.1% of the dry weight, the highest in the shoot is only 0.16% of the dry weight, and artemisinin is almost undetectable in the root, so tissue culture is used to produce Artemisia annua Artemisinin less likely
The use of genetic engineering to increase the content of artemisinin in Artemisia annua is still in the trial stage, and is subject to strict restrictions on the safe production of genetically modified products by the state, so it is predicted that it will be difficult to achieve commercial production in the next 3-5 years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Effects of different proportions of composition on increasing the content of artemisinin in Artemisia annua

[0012] The seeds of Artemisia annua (from the Artemisia annua species with high artemisinin content in Xiangxi, Hunan) are sprayed with water to moisten the seedbed one day before sowing. When sowing, the seeds are mixed into seed ash, spread evenly on the border surface, and then covered with a thin layer of plant ash to cover The seedlings will emerge within 10-15 days. After emergence, thinning is carried out, and the seedlings are kept at a distance of about 10cm. When the A. annua seedlings grow to 10-15cm, transplant them and plant them in the field. Before transplanting, combined with deep plowing and deep ploughing, apply 50 kg of nitrogen, phosphorus, and potassium compound fertilizer, and pour enough root water after planting. Dig holes in the borders of 60 cm × 70 cm row spacing, plant 1 plant per hole and 1500 plants per mu.

[0013] In the early sta...

Embodiment 2

[0018] Preparation method of composition for increasing artemisinin content in artemisia annua

[0019] Take 13.22mg of abscisic acid (molecular weight 264.32) and dissolve in 500ml water; take 75mg of chitosan (molecular weight 1500) and dissolve in 500ml water; mix the two to make the final concentration of abscisic acid and chitosan reach 50μM; After adding 0.05% (w / v) Tween-20 to the mixture, place it on a shaker at room temperature and mix at 50rpm for 10-20min.

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PUM

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Abstract

The invention belongs to the technical field of biologics, and particularly relates to a composition for increasing artemisinin content in artemisia apiacea and a preparation method thereof. The composition is prepared from the following components of abscisic acid and chistan according to a concentration ratio (mu M) of (1 to 100) to (100 to 1). The preparation method of the composition comprises the following steps of respectively dissolving the abscisic acid and the chista into water, and mixing, so as to reach the final concentration ratio; adding Tween-20 into the mixed liquid, and uniformly mixing, so as to obtain the composition. The composition has the advantages that the synergistic action of the components is realized, and the artemisinin content in artemisia apiacea is obviously increased.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a composition for increasing the content of artemisinin in Artemisia annua and a preparation method thereof. Background technique [0002] Malaria seriously affects human health, causing millions of deaths every year, and is known as the number one "killer" in Africa. Artemisinin (artemisinin, a sesquiterpene lactone with a new structure) extracted from the Compositae plant Artemisia annua L. native to my country is currently the most effective antimalarial drug, with artemisinin as the main raw material Artemisinin-Combined Therapy (Artemisinin-Combined Therapy) is recommended by the World Health Organization as the primary treatment for malaria, especially falciparum malaria. The global annual demand for artemisinin is huge, about 140 tons / year, and it is growing at a rate of 10-20% per year. Artemisinin is mainly derived from the aerial parts of A. annua plants. However, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N37/42A01P21/00A01G1/00A01G7/06
CPCA01N43/16A01G7/06A01G22/00A01N37/42
Inventor 谢兵秦检
Owner CHONGQING DINGSHENG BIOTECH CO LTD
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