A rapid non-thermal preparation method of rare ginsenoside rg3(s)

Abstract

The invention relates to a method for rapid athermal preparation of rare ginsenoside Rg3 (S), and particularly relates to a preparation method of high-voltage pulsed electric field technology coupling acid hydrolysis. The technical scheme is as follows: at room temperature, evenly mixing an acidic ginsenoside Rb1 solution, directly applying a high-pressure pulsed electric field for processing with the electric field strength being 5-25 kV / cm, the processed pulse number being 1-15 and the acid concentration being 0.1-2%; and adding a material processed by the high-pressure pulsed electric field into an organic solvent for centrifugal separation, and taking a supernatant to remove the solvent by volatilization to obtain the ginsenoside Rg3 (S). According to the method, the high-voltage pulsed electric field technology is introduced in the acid hydrolysis process of ginsenoside Rb1 to accelerate the reaction speed of ginsenoside Rb1 hydrolysis, and rapidly and efficiently promote the ginsenoside Rb1 to convert into the rare ginsenoside Rg3 (S). . Compared with the traditional methods, the method is free of heating, short in preparation time, high in yield, less in by-products and low in energy consumption.

Description

technical field

[0001] The invention relates to a method for rapidly and non-thermally preparing rare ginsenoside Rg3(S), in particular to a preparation method coupled with high-voltage pulsed electric field technology and acid hydrolysis. Background technique

[0002] Ginseng (Panax ginseng C.A. Meyer) is a plant of the genus Panax in the Araliaceae family. It has a variety of pharmacological activities, including lowering blood lipids, immune regulation, anti-inflammatory effects, chemopreventive effects, anti-aging, anti-oxidation and other functions. Ginsenoside is one of the main pharmacological components of ginseng, and more than 80 kinds of ginsenoside monomers have been isolated and identified from ginseng.

[0003] Ginsenoside Rg3 has two optical isomers (S-type and R-type) (Fig. 1), which have different physiological and biological activities respectively, and the water solubility and biological activity of ginsenoside Rg3(S) are higher than those of the isomer Rg...

Examples

Embodiment 1

[0015] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in 1% hydrochloric acid solution, prepare a solution with a concentration of 1mM ginsenoside Rb1, and directly pass it into a high-voltage pulsed electric field at normal temperature Treat, its electric field intensity is 20kV / cm, and pulse number is 10; Water saturated n-butanol extraction reaction liquid, centrifugation, supernatant liquor volatilizes and removes solvent, and the yield of ginsenoside Rg3 (S) is analyzed by high performance liquid chromatography It was 68.58%.

Embodiment 2

[0017] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in a 1.5% hydrochloric acid solution, prepare a 1mM ginsenoside Rb1 solution, and directly pass it into a high-voltage pulsed electric field at room temperature To process, its electric field strength is 25kV / cm, and the number of pulses is 10; water-saturated n-butanol extracts the reaction solution, centrifuges, the supernatant is volatilized to remove the solvent, and the yield of ginsenoside Rg3 (S) is analyzed by high performance liquid chromatography It was 63.28%.

Embodiment 3

[0019] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in a 1.5% hydrochloric acid solution, prepare a 1mM ginsenoside Rb1 solution, and directly pass it into a high-voltage pulsed electric field at room temperature To process, its electric field intensity is 20kV / cm, and the number of pulses is 8; the reaction solution is extracted with water-saturated n-butanol, centrifuged, the supernatant is volatilized to remove the solvent, and the yield of ginsenoside Rg3(S) is analyzed by high performance liquid chromatography It was 54.47%.

technical field

[0001] The invention relates to a method for rapidly and non-thermally preparing rare ginsenoside Rg3(S), in particular to a preparation method coupled with high-voltage pulsed electric field technology and acid hydrolysis. Background technique

[0002] Ginseng (Panax ginseng C.A. Meyer) is a plant of the genus Panax in the Araliaceae family. It has a variety of pharmacological activities, including lowering blood lipids, immune regulation, anti-inflammatory effects, chemopreventive effects, anti-aging, anti-oxidation and other functions. Ginsenoside is one of the main pharmacological components of ginseng, and more than 80 kinds of ginsenoside monomers have been isolated and identified from ginseng.

[0003] Ginsenoside Rg3 has two optical isomers (S-type and R-type) (Fig. 1), which have different physiological and biological activities respectively, and the water solubility and biological activity of ginsenoside Rg3(S) are higher than those of the isomer Rg...

Examples

Embodiment 1

[0015] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in 1% hydrochloric acid solution, prepare a solution with a concentration of 1mM ginsenoside Rb1, and directly pass it into a high-voltage pulsed electric field at normal temperature Treat, its electric field intensity is 20kV / cm, and pulse number is 10; Water saturated n-butanol extraction reaction liquid, centrifugation, supernatant liquor volatilizes and removes solvent, and the yield of ginsenoside Rg3 (S) is analyzed by high performance liquid chromatography It was 68.58%.

Embodiment 2

[0017] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in a 1.5% hydrochloric acid solution, prepare a 1mM ginsenoside Rb1 solution, and directly pass it into a high-voltage pulsed electric field at room temperature To process, its electric field strength is 25kV / cm, and the number of pulses is 10; water-saturated n-butanol extracts the reaction solution, centrifuges, the supernatant is volatilized to remove the solvent, and the yield of ginsenoside Rg3 (S) is analyzed by high performance liquid chromatography It was 63.28%.

Embodiment 3

[0019] The method for degrading ginsenoside Rb1 to generate ginsenoside Rg3(S) in the present invention is to dissolve the ginsenoside Rb1 sample in a 1.5% hydrochloric acid solution, prepare a 1mM ginsenoside Rb1 solution, and directly pass it into a high-voltage pulsed electric field at room temperature To process, its electric field intensity is 20kV / cm, and the number of pulses is 8; the reaction solution is extracted with water-saturated n-butanol, centrifuged, the supernatant is volatilized to remove the solvent, and the yield of ginsenoside Rg3(S) is analyzed by high performance liquid chromatography It was 54.47%.

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