Method for distinguishing salvia miltiorrhiza medicinal material or derivative

A derivative, Danshen technology, applied in the field of identification of natural medicinal plants, can solve the problems of ionization degree and matrix interference, poor repeatability and feasibility, and difficult to analyze

Active Publication Date: 2014-03-26
王顺才
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the mainstream method HPLC fingerprint is affected by non-chromatographic conditions (such as chromatographic column inner diameter, length, stationary phase grade, carrier particle size, mobile phase flow rate, proportion of components in mixed mobile phase, column temperature, injection volume, detector sensitivity, etc.) There are many limitations in repeatability and feasibility due to the large impact and the need for standard products; although the HPCE method is superior to the HPLC method because of its high efficiency, rapidity, simplicity, and less contamination of the column, there are still reproducibility Poor, narrow linear range and low sensitivity; a single LC-MS analysis method is usually not enough for a variety of Accurate structural characterization of components; the IR acquisition process is greatly affected by environmental changes and is not easy to analyze, and the repeatability and feasibility are poor

Method used

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  • Method for distinguishing salvia miltiorrhiza medicinal material or derivative
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  • Method for distinguishing salvia miltiorrhiza medicinal material or derivative

Examples

Experimental program
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preparation example Construction

[0165] a) Preparation of Danshen medicinal material or decoction pieces for test solution

[0166] Accurately weigh the test product: 0.4~0.5g of the powder of Danshen medicinal material / decoction pieces, add 25mL of 75% methanol, heat to reflux for 1 hour, filter, and concentrate the filtrate to 1mL, and use it as the test solution of Danshen medicinal material or decoction pieces.

[0167] b) Preparation of the test solution of other Danshen derivatives

[0168] Accurately weigh the test product: 200mg of other Danshen derivatives (such as salvianolic acid extract) in a 100mL volumetric flask, add an appropriate amount of methanol (30-40mL) to dissolve, dilute to the mark after ultrasonic oscillation, and shake well to get other Danshen Derivatives test solution (such as salvianolic acid extract test solution).

[0169] iv) Assay

[0170] Accurately draw 20 μl each of the salvianolic acid B standard reference solution and the test solution containing salvianolic acid activ...

Embodiment 1

[0260] Example 1: Henan Luoyang Danshen medicinal material IGD carbon NMR fingerprint

[0261] (1) Preparation of characteristic extracts

[0262] Select Danshen medicinal material in Luoyang, Henan Province, and prepare Danshen medicinal material tanshinone and

[0263] Salvianolic acid characteristic extract:

[0264] 1) The preparation method of the characteristic extract of tanshinone is as follows: take the root and bark of Danshen that have been dried in the shade, pulverize, accurately weigh, and extract twice with 6 times the volume of 95v% ethanol under reflux, combine the filtrates, concentrate under reduced pressure, recover the solvent and evaporate Dried to powder, the 95v% ethanol extract of Salvia miltiorrhiza is obtained. Take the above powder (Salvia miltiorrhiza 95v% ethanol extract) and add 10 times the volume of ethyl acetate, ultrasonically extract at 55°C for 20 minutes, centrifuge, add 10 times the volume of ethyl acetate for precipitation, and ultraso...

Embodiment 2

[0291] Example 2: Central Plains Zhengxin Danshen Medicinal Material IGD Carbon NMR Fingerprint

[0292] (1) Preparation of characteristic extracts

[0293] Select the medicinal material of Danshen from Zhongyuan Zhengxin, and prepare the characteristic extracts of tanshinone and salvianolic acid from the medicinal material of Danshen according to the following method:

[0294] 1) The preparation method of the characteristic extract of tanshinone is as follows: take the root and skin of Danshen that have been dried in the shade, pulverize, accurately weigh, extract 3 times with 10 times the volume and 95v% ethanol, combine the filtrates, concentrate under reduced pressure, recover the solvent and evaporate to dryness To powder, the 95v% ethanol extract of Danshen medicinal material is obtained. Take the above powder (95v% ethanol extract of Salvia miltiorrhiza) and add 10 times the volume of ethyl acetate, reflux extraction at 75°C for 20 minutes, centrifuge, add 10 times the...

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Abstract

The invention relates to a method for distinguishing a salvia miltiorrhiza medicinal material or a derivative. The method comprises the following steps: (1) extracting the salvia miltiorrhiza medicinal material or the derivative, to obtain a tanshinone characteristic extract and a danshinolic acid characteristic extract which contain active component groups; (2) performing IGD nuclear magnetic resonance carbon spectrum finger print detection on both the tanshinone characteristic extract and the danshinolic acid characteristic extract, obtaining the peak intensity of characteristic peaks of a plurality of active components in the characteristic extracts according to the finger print; and detecting the peak intensity of the characteristic peak of corresponding standard reference of each active component with the same manner; (3) obtaining the absolute content of the standard references by a quantitative analysis measure; and (4) calculating the content of each active component and the content of the active component groups in the salvia miltiorrhiza medicinal material or the derivative by utilizing the ratio of the peak intensities of the characteristic peaks and the absolute content. Due to the adoption of the method, the tanshinone and danshinolic acid compounds contained in the salvia miltiorrhiza and the ratio of the tanshinone compound to the danshinolic acid compound can be indicated, and the purpose of realizing quality determination of salvia miltiorrhiza medicinal material or derivative is achieved.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identification of salvia miltiorrhiza or derivatives. Background technique [0002] Salvia miltiorrhiza Bge. is the dry root and rhizome of Salvia miltiorrhiza Bge., which is one of the commonly used traditional Chinese medicines in my country. Salvia miltiorrhiza guixin, liver meridian, slightly cold in nature, bitter in taste, non-toxic, has the effects of dispelling addiction and relieving pain, promoting blood circulation and regulating menstruation, nourishing the heart and eliminating troubles. Its active ingredients can be divided into fat-soluble ingredients and water-soluble ingredients. The fat-soluble components are mainly tanshinone compounds (mainly o-quinones, diterpene quinones and their derivatives) compounds, which have the effects of dilating blood vessels, improving microcirculation, and antibacterial and anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08A61K36/537
Inventor 张海艳赵天增董建军陈荣峰
Owner 王顺才
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