A kind of ivermectin chemiluminescent ELISA rapid detection kit

Abstract

The invention relates to an ivermectin detection kit, in particular to an ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit. The ivermectin chemiluminescence enzyme-linked immunoassay rapid detection kit comprises a kit body, an elisa plate arranged in the kit body and a reagent filled in the kit body, wherein each hole of the elisa plate is coated with coating antigens, the coating antigens are prepared by coupling metabolites of ivermectin with ovalbumin; the reagent comprises monoclonal antibodies of the ivermectin, elisa secondary antibodies, namely horseradish peroxidase-marked goat-anti-mouse antibodies, ivermectin series of standard solutions, concentrated phosphate buffer liquid, concentrated washing liquid and luminescent liquid. By using the kit, due to the adoption of the steps of adding a sample, washing, adding the elisa secondary antibodies, washing, adding the luminescent liquid, detecting, and judging results, the prepared kit has high sensitivity which can reach 0.01 to 5 ng / ml and has a wide linear dynamic range; an analysis method is simple, convenient and quick, the results are stable, and the error is small; the analysis results are sensitive, stable and reliable; the safety is high; the using period is long.

Description

technical field [0001] The invention relates to a detection kit for ivermectin, in particular to a rapid detection kit for ivermectin chemiluminescent ELISA. Background technique [0002] After the 1990s, the vast majority of physical and chemical detection methods for the determination of ivermectin drug residues in food mainly rely on liquid chromatography for separation, followed by chromatography / mass spectrometry, gas chromatography, and high-performance thin-layer chromatography. And other detection methods, because of their unique performance, have a little application in the detection of antibiotic residues. Chromatographic detection of drug residues in milk requires sample processing (including sample extraction, deproteinization, centrifugation, chromatographic column purification, derivatization, etc.), separation of residual drugs, and detection of residual drugs. The physical and chemical detection method uses the special reaction or property of the group in th...

AI Technical Summary

Problems solved by technology

[0002] After the 1990s, the vast majority of physical and chemical detection methods for the determination of ivermectin drug residues in food mainly rely on liquid chromatography for separation, followed by chromatography / mass spectrometry, gas chromatography, and high-performance thin-layer chromatography. and other detection methods, because of their unique performance, have a little application in the detection of antibiotic residues

However, because antibiotics are haptens, the antigen construction technology is difficult, the immune specificity is strong, and the detection cost is high. At present, IDEXX Laboratory Company, Chahu Sciences and other companies have relatively mature research on the free analysis method, and this technology is still in research and development in China. middle

At present, enzyme-linked immunosorbent assay, radioimmunoluminescence assay, fluorescence immunoassay, chemiluminescence method, etc. are mainly used. Comparing the sensitivity of several existing marker immunological techniques, it can be seen that enzyme immunoassay has a lower sensitivity than luminescence.